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Using a barcoded AAV capsid library to select for clinically relevant gene therapy vectors

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Figure

Figure 1. Evaluation of parental capsids for islet tropism. (with AAV2, AAV3B, DJ, and LK03 capsids at a MOI of 1,000, and transduction efficiency was determined by flow cytometry 48 hours after transduc-A) Dissociated islet cells were transduced with diff
Figure 2. Enrichment of distinct capsids during passaging. Barcode sequences were amplified from viral genomes after each passage and were analyzed by high-throughput sequencing
Figure 3. Rescue of enriched capsid sequences and evaluation of selected capsids for islet transduction
Figure 4. Analysis of transduction efficiency of the variants. (A) GFP-expressing rAAV packaged with 2 of the variant capsids, as well as AAV-DJ and AAV-LK03 capsids, were used to transduce intact islets from 2 individual donors (donors A and B) at a MOI o
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