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Observation weights unlock bulk RNA seq tools for zero inflation and single cell applications

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Figure

Fig. 1 Zero inflation results in overestimated dispersion and jeopardizes power to discover differentially expressed genes.counts per million (CPM) computed usingobserved also for the real scRNA-seq data in panels (zeros are downweighted in dispersion esti
Fig. 2 Impact of zero inflation on mean–variance relationship for simulated bulk RNA-seq and Islam scRNA-seq datasets
Figure 3 (Additional file 1: Figure S7) illustrates thatmany methods break down on the simulated Islam datasetdue to a high degree of zero inflation
Figure S9). Note that the remaining methods also sufferfrom poor FDR control.Since zero inflation is fairly modest for the Trapnell
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