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Colon Adenocarcinoma Diagnosis in Human Samples by Multicontrast Nonlinear Optical Microscopy of Hematoxylin and Eosin Stained Histological Sections

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Figure

Figure 1. Schematic view of multimodal NLO setup. In black letters principal components (laser, mi-croscope, and detectors) and in color letters different nonlinear signal collected are indicated
Figure 2. Multicontrast representative in face imaging (512 × 512 pixels) of normal colon tissue
Figure 3. Representative TPEF (green) + SHG (red) in face imaging at a two-photon excitation wavelength of 940 nm of normal and adenocarcinoma colonic mucosa
Figure 4. (A) Representative TPEF (green) + THG (magenta) in face imaging (512 × 512 pixels) at two-photon excitation wavelength of 940 nm of normal and adenocarcinoma colonic mucosa
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