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Glutaminase 1 plays a key role in the cell growth of fibroblast like synoviocytes in rheumatoid arthritis

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Figure

Fig. 1 RA-FLS exhibit higher HK2, MCT4, PDK1, and GLS1 mRNA levels than OA-FLS. Glycolysis- and glutaminolysis-related mRNAs were examined in 12 OA-FLS and 19 RA-FLS by real-time PCR, and their levels were normalized to that of GAPDH mRNA
Fig. 3 Glutamine is required for the proliferation of RA-FLS. a RA-FLS proliferation was determined using the BrdU assay 96 h after transfection with HK2, MCT4, PDK1, GLS1, or SC siRNA ( n = 5)
Fig. 4 GLS1 is upregulated in RA-FLS. a GLS1 protein levels in 4 OA-FLS and 4 RA-FLS were determined by immunoblotting
Fig. 5 Compound 968, a GLS1 inhibitor, suppresses the proliferation of RA-FLS. a RA-FLS were cultured with or without compound 968 and quantified using the WST-8 assay ( n = 3)

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