Leukocyte Typing II
VOLUME 2
Leukocyte Typing II
Volume 1 Human T Lymphocytes
Volume 2 Human B Lymphocytes
Leukocyte Typing
II
VOLUME 2
Human B Lymphocytes
Edited by
Ellis L. Reinherz Barton F. Haynes
Lee M. Nadler Irwin D. Bernstein
With 134 Illustrations
Springer -Verlag
EUis L. Reinherz, M.D., Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115 U.S.A.
Barton F. Haynes, M.D., Department of Medicine, Duke University School of Medicine, Durham, NC 27710 U.S.A.
Lee M. Nadler, M.D., Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115 U.S.A.
Irwin D. Bernstein, M.D., Program in Pediatric Oncology, Fred Hutchinson Can-cer Research Center, Seattle, WA 98104 U.S.A.
Library of Congress Cataloging in Publication Data Main entry under title:
Leukocyte typing II.
Papers presented at the Second International
Workshop on Human Leukocyte Differentiation Antigens, held in Boston, Sept. 17-20, 1984.
Includes bibliographies and indexes.
Contents: v. I. Human T lymphocytes-v. 2. Human B lymphocytes-v. 3. Human myeloid and hematopoietic cells.
I. Leucocytes-Classification-Congresses. 2. Histocompatibility testing-Congresses. 3. Tissue specific antigens-Analysis-Congresses. I. Reinherz, Ellis L. II. International Workshop on Human
Leukocyte Differentiation Antigens (2nd: 1984 : Boston, Mass.) III. Title: Leukocyte typing 2. IV. Title:
Leukocyte typing two.
QRI85.8.L48L48 -1985 616.07'9 85-22229 © 1986 by Springer-Verlag New York Inc.
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Typeset by Bi-Comp Inc., York, Pennsylvania.
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ISBN-13: 978-1-4612-9328-6 e-ISBN-13: 978-1-4612-4848-4 DOl: 10.1007/978-1-4612-4848-4
Preface
The Second International Workshop on Human Leukocyte Differentia-tion Antigens was held in Boston, September 17-20, 1984. More than 350 people interested in leukocyte differentiation agreed to exchange reagents and participate in this joint venture. All in all, in excess of 400 antibodies directed against surface structures on T lymphocytes, B lymphocytes, and myeloid-hematopoietic stern cells were characterized. Because of the enormous quantity of serologic, biochemical, and functional data,
Leuko-cyte Typing II has been divided into three volumes.
These books represent the written results of workshop participants. They should be helpful to both researchers and clinicians involved in scientific endeavors dealing with these broad fields of immunobiology. To those who delve into the various sections of the volumes, it will become evident that the work speaks for itself.
I am deeply indebted to the section editors, Barton F. Haynes, Volume
1, Human T Lymphocytes, Lee M. Nadler, Volume 2, Human B
Lympho-cytes, and Irwin D. Bernstein, Volume 3, Human Myeloid and
Hemato-poietic Cells for their major contributions in planning, executing, and summarizing the workshop, as well as council members John Hansen, Alain Bernard, Laurence Boumsell, Walter Knapp, Andrew McMichael, Cesar Milstein, and Stuart F. Schlossman. I would also like to thank the National Institutes of Health, World Health Organization, and Interna-tional Union of Immunological Societies for making this meeting possible. Needless to say, I am most grateful to all of my colleagues who contrib-uted to this effort and helped to accelerate the characterization of human immunobiology through their endeavors.
Contents
I. Introduction
1. B Cell/Leukemia Panel Workshop: Summary and Comments
L.M. Nadler 3
II. Serologic Specificity of B Cell/Leukemia Monoclonal
Anti-bodies
2. Analysis of the B Cell/Leukemia Workshop Monoclonal Antibodies Using an Immunoenzymatic Staining Assay and a Radioimmunoassay on Cells B. Darken,
G. Moldenhauer, A. Pezzutto, R. Schwartz, S. Kiesel,
W. Hunstein 47
3. Analysis of Ten B Lymphocyte-Specific Workshop Monoclonal Antibodies G. Moldenhauer, B. Darken,
R. Schwartz, A. Pezzutto, J. Knops, GJ. Hiimmerling 61
4. Screening of Workshop "B" Series Antibodies by
Radioimmunobinding to Human Leukocyte Cell Lines and to Cells from Human Lymphoid Tumors C.M. Steel,
P. Elder, K. Guy 69
5. Expression of Lymphocyte Differentiation Antigens in Immunodeficiency Diseases T.F. Tedder, L.T. Clement,
MJ. Crain, M.D. Cooper 79
6. Use of Two Monoclonal Anti-Human B Cell Antibodies in the Study of Early B Cell Differentiation A. Raghavachar,
S.F. Goldmann, B. Kubanek 87
7. Characterization of a Human B Lymphocyte-Specific Antigen Defined by Monoclonal Antibodies HD6 and
viii Contents
HD39 G. Moldenhauer, B. Darken, R. Schwartz,
A. Pezzutto, GJ. Hiimmerling 97
8. Six Distinct Antigen Systems of Human B Cells as Defined by Monoclonal Antibodies Y. Ishii, T. Takami, H. Yuasa,
T. Takei, Y. Kokai, K. Kikuchi 109
9. B-Cl, B-C2, B-C3: Monoclonal Antibodies against B Cell Differentiation Antigens I. Anegon, R. Vi/ella, T. Gallart,
C. Cuturi, L. Borche, J. Mila, J. Vives 121
10. Human B Cells: Is FMC7 a Marker for Relatively Mature B Cells or Does It Define a Population Equivalent to the
LyB5-Negative Mouse B Cells? H. Zola 141
11. Spontaneous Mouse Erythrocyte Rosette Formation
(M-RFC) with Human B Lymphocytes: Diagnostic Value in B-Lymphoproliferative Diseases and Lack of Relationship to B Cell Protocol Monoclonal Antibodies P. Hokland, K. Meyer, I.M. Fastrup
III. Biochemical Analysis of Antigens Defined by the Workshop B Cell/Lenkemia Panel
12. Human B Cell Surface Molecules Defined by an Interna-tional Workshop Panel of Monoclonal Antibodies
147
E.A. Clark, D. Einfeld 155
13. Structural Analysis of Cell Surface Molecules Recognized by Leukemic Cell/B Cell Panel Antibodies T. W. LeBien,
J.G. Bradley, J.L. Platt, SJ. Pirruccello 169
14. Biochemical Analysis of Antigens Recognized by Workshop B Series Antibodies, Using "Western Blotting"
B.B. Cohen, M. Moxley, P. Elder, K. Guy, C.M. Steel 177
15. Human B Cell Antigens Detected by the Workshop
Antibodies: A Comparison of Serological and Immunochem-ical Patterns K. Horibe, R. W. Knowles 187
16. Further Evidence that the Human Differentiation Antigen p24 Possesses Activity Associated with Protein Kinase
T.F. Zip/, G.R. Antoun, G.J. Lauzon, B.M. Longenecker 203
17. Clustering of Anti-Leukemia and Anti-B Cell Monoclonal
Antibodies A.M. Ravoet, A.M. Lebacq-Verheyden 213
18. A Structurally Novel Human B Cell Surface Molecule
Contents ix
19. Human Neutrophils Synthesize Different Forms of the Common Acute Lymphoblastic Leukemia Antigen
R.T. McCormack, J.G. Bradley, T. W. LeBien 235
IV. Immunohistochemical Analysis of B Cell/Leukemia Panel Monoclonal Antibodies
20. Immunohistochemical Analysis of Monoclonal Anti-B Cell
Antibodies D. Y. Mason, H. Ladyman, K.C. Gatter 245
21. Analysis of Band L Workshop Antibodies on Sections of Normal and Neoplastic Lymphoid Tissue and Cell Lines
I.C.M. MacLennan, P.D. Nathan, G.D. Johnson, M. Khan, L. Walker, N.R. Ling 257
22. Immunohistol9gical Analysis of Tissue Specificity of the Fifty-two Workshop Anti-B Lymphocyte Monoclonal
Antibodies G. Pallesen 277
23. Reactivity of Monoclonal Antibodies of the Band L Series with Follicular Dendritic Cells in Tissue Sections and with Lymphoblastoid Cell Lines G.D. Johnson,
I.C.M. MacLennan, N.R. Ling, D.L. Hardie,
P.D. Nathan, L. Walker 289
24. The Staining of a Panel of Routine Diagnostic Tissue Biopsies with the Workshop "L" Series Antibodies
D.B. Jones, K.M. Britten, D.H. Wright
25. Immunohistochemical Reactivity of Anti-B Cell Monoclonal Antibodies in Thymus, Lymph Node, and Normal Skin
E. Berti, C. Parravicini, G. Cattoretti, D. Delia,
299
F. de Braud, M. Cusini 313
26. Report to Second International Workshop on Human Leukocyte Differentiation Markers: Boston, 1984. Tissue Localization of B Cell and Leukemic Reagents (Special Studies) N. Kraft, P.S. Giddy, W. W. Hancock,
R.C. Atkins 319
V. Expression of B Cell/Leukemia Panel on Leukemias and Lymphomas
27. Quantitative Phenotypes of B Chronic Lymphocytic Leukemia B Cells Established with Monoclonal Antibodies from the B Cell Protocol P. Poncelel,
x Contents
28. Analysis of Workshop "L" Series Antibodies: Radioimmunobinding and Biochemical Studies
C.M. Steel, B.B. Cohen, P. Elder, M. Moxley, K. Guy 345
29. Subclassification of Leukemia Using Monoclonal Antibodies G.R. Pilkington, G.T.H. Lee,
P.M. Michael, O.M. Garson, N. Kraft, R.C. Atkins, D.G. Jose
30. Immunological Classification of "Unclassifiable" Acute Leukemia F. Herrmann, B. Darken, A. Gatzke,
353
W.D. Ludwig 367
31. Age Predilection of Distinct Phenotypically Defined Subgroups of N on-T Cell ALL: Studies Using B
Cell-Restricted or -Associated Monoclonal Antibodies in 359 Patients P. Herrmann, B. Darken, W.D. Ludwig,
H. Riehm 377
32. Heterogeneity of B-CLL Cells Defined by Monoclonal Antibodies N. Genetet, D. Bourel, B. Grosbois,
G. Merdrignac, M. Marty, R. Fauchet, F. Lancelin,
R. Leblay, B. Genetet 385
33. HD37 Monoclonal Antibody: A Useful Reagent for Further Characterization of "Non-T, Non-B" Lymphoid Malignancies A. Pezzutto, B. Darken, A. Feller,
G. Moldenhauer, R. Schwartz, P. Wernet, E. Thiel,
W. Hunstein 391
34. Characterization of Burkitt's Lymphoma Cell Lines with Monoclonal Antibodies Using an ELISA Technique
J.T. Sandlund, J. Kiwanuka, G.E. Marti, W. Goldschmidts,
I. T. Magrath 403
VI. Functional Significance of Human B Cell/Leukemia Antigens
35. Human B Cell Populations Defined by the Bl and B2 Antigens K.C. Anderson, A. W. Boyd, D.C. Fisher,
J.F. Daley, S.P. Schlossman, L.M. Nadler 413
36. Phenotypic Changes Occurring during in vitro Activation of
Human Splenic B Lymphocytes A. W. Boyd, A.S. Freedman, K.C. Anderson, D.C. Fisher,
J.C. Horowitz, J.P. Daley, S.F. Schlossman, L.M. Nadler 429
37. Antigenic Changes of the B Cell Panel of the Second International Workshop on Human Leukocyte Differentiation Antigens with in vitro Activation
Contents xi
A.S. Freedman, A. W. Boyd, D.C. Fisher, S.F. Schlossman,
L.M. Nadler 443
38. Activation of Human B Cells with Monoclonal Antibody to the Bp32 Cell Surface Polypeptide E.A. Clark, G. Shu,
J.A. Ledbetter 455
39. B Lymphocyte Surface Antigens Involved in the Regulation of Immunoglobulin Secretion J. Golay, F. Rawle,
P. Beverley 463
40. Inhibition of Immunoglobulin Secretion, But Not Immunoglobulin Synthesis, by a Monoclonal Antibody
S. Pittaluga, J. Cossman, J.B. Trepel, L.M. Neckers 473
41. Monoclonal Antibody AB1 Identifies a Human B Cell Activation Antigen and Inhibits Growth Factor-Dependent Human B Cell Proliferation L.K.L. Jung, S.M. Fu,
J. Morgan, A.L. Maizel 483
42. Detection and Functional Studies of IL-2 Receptors on Activated Human B Cells L.K.L. Jung, T. Hara,
S.M. Fu 491
43. The C3d Receptor Identified by the HB-5 Monoclonal Antibody: Expression and Role as a Receptor for
Epstein-Barr Virus T.F. Tedder, L.T. Clement, J.J. Weis,
D.T. Fearon, M.D. Cooper 499
44. The B Cell-Restricted Glycoprotein (B2) Is the Receptor for Epstein-Barr Virus L.M. Nadler, A. W. Boyd, E. Park,
K.C. Anderson, D.C. Fisher, B. Slaughenhoupt,
D.A. Thorley-Lawson, S.F. Schlossman 509
45. Monoclonal Antibody-Defined Cell Surface Molecules Regulate Lymphocyte Activation D.R. Howard,
A.C. Eaves, F. Takei 519
46. TPA-Induced Modulation of B Cell Differentiation Antigens Defined by Monoclonal Antibodies (HD6, HD28, HD37, HD39) R. Schwartz, G. Moldenhauer, B. Darken,
A. Pezzutto, F. Momburg, V. Schirrmacher 527
47. Functional Studies of p24: Platelet Aggregation Inhibition by Fab Monomers of BA-2 J.E. Brown, J.G. White,
R.D. Hockett, Jr., K.R. Hagert, J.H. Kersey 541
Contributors
Kenneth C. Anderson Division of Tumor Immunology, Dana-Farber
Cancer Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Ignacio Anegon Servicio de Inmunologfa, Hospital Clfnic i Provincial,
08036 Barcelona, Spain
Gamil R. Antoun Department of Pediatrics and the Tom Baker Cancer
Centre, University of Calgary, Calgary, Alberta, Canada T2N 4N1
Robert C. Atkins Nephrology Department, Prince Henry's Hospital,
Melbourne, Australia
Emilio Berti First Department of Dermatology, University of Milan,
Milan, Italy
Peter Beverley ICRF Human Tumor Immunology Group; School of
Medicine, University College London, London WClE 6JJ, U.K.
Luis Borche Servicio de Immunologfa, Hospital Clinic i Provincial,
08036 Barcelona, Spain
D. Bourel Groupe Universitaire de Recherche en Immunologie
Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
Andrew W. Boyd Division of Tumor Immunology, Dana-Farber Cancer
Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115 U.S.A.
J. Garrett Bradley Department of Laboratory Medicine/Pathology, U ni-versity of Minnesota Medical School, Minneapolis, Minnesota 55455, U.S.A.
K.M. Britten University Department of Pathology, General Hospital
Southampton S09 4XY, U.K.
Jo Ellen Brown University of Minnesota, Minneapolis, Minnesota
55455, U.S.A.
Pierre Carayon Immunotoxin Project, Research Center Clin-Midy,
xiv Contributors
Giorgio Cattoretti National Cancer Institute of Milan, Milan, Italy Edward A. Clark Department of Microbiology and Immunology,
Uni-versity of Washington, Seattle, Washington 98195, U.S.A.
Loran T. Clement Cellular Immunobiology Unit, Tumor Institute,
Uni-versity of Alabama in Birmingham, Birmingham, Alabama 35294, U.S.A.
B.B. Cohen Medical Research Council, Clinical and Population
Cytoge-netics Unit, Western General Hospital, Edinburgh, U.K.
Max D. Cooper Cellular Immunobiology Unit, Tumor Institute,
Univer-sity of Alabama in Birmingham, Birmingham, Alabama 35294, U.S.A.
Jeffrey Cossman Laboratory of Pathology, National Cancer Institute,
National Institutes of Health, Bethesda, Maryland 20205, U.S.A.
Marilyn J. Crain Cellular Immunobiology Unit, Tumor Institute,
Uni-versity of Alabama in Birmingham, Birmingham, Alabama 35294, U.S.A.
Marco Cusini First Department of Dermatology, University of Milan,
Milan, Italy
Cristina Cuturi Servicio de Inmunologfa, Hospital Clinic i Provincial,
08036 Barcelona, Spain
John F. Daley Division of Tumor Immunology, Dana-Farber Cancer
Inl'titute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Filippo de Braud National Cancer Institute of Milan, Milan, Italy Domenico Delia National Cancer Institute of Milan, Milan, Italy Bernd Dorken Medizinische U niversitats-Poliklinik, D-6900
Heidel-berg, F.R.G.
Allen C. Eaves Terry Fox Laboratory, B.C. Cancer Research Centre,
Vancouver, British Columbia, Canada
David Einfeld Immunobiology Group, Genetic Systems Corporation,
Seattle, Washington 98121, U.S.A.
Patricia Elder Medical Research Council Clinical and Population
Cyto-genetics Unit, Western General Hospital, Edinburgh, U.K.
lise Marie Fastrup The University Department of Medicine and
Hema-tology, Aarhus Amtssygehus, Denmark DK-8000
R. Fauchet Groupe Universitaire de Recherche en Immunologie
Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
Douglas T. Fearon Department of Medicine, Harvard Medical School,
Department of Rheumatology & Immunology, Brigham and Wom-en's Hospital, Boston, Massachusetts 02115, U.S.A.
A. Feller Pathologisches Institute der Universitat Kiel, KieI, F.R.G. David C. Fisher Division of Tumor Immunology, Dana-Farber Cancer
Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Contributors xv Arnold S. Freedman Division of Tumor Immunology, Dana-Farber Can-cer institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Shu Man Fu Cancer Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, U.S.A.
Teresa Gallart Servicio de Inmunologfa, Hospital Clinic i Provincial, 08036 Barcelona, Spain
O. Margaret Garson Immunogenetics Research Unit, Cancer Institute, Melbourne, Australia 3000
K.C. Gatter Department of Haematology, John Radcliffe Hospital, Ox-ford OX3 9DU, U.K.
A. Gatzke Abt. Hamatologie, Klinikum Steglitz, Freie Universitat Berlin, Berlin, F.R.G.
B. Genetet Groupe Universitaire de Recherche en Immunologie Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
N. Genetet Groupe Universitaire de Recherche en Immunologie Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
Peter S. Giddy Nephrology Department, Prince Henry's Hospital, Mel-bourne, Australia
Josee Golay ICRF Human Tumor Immunology Group, School of Medi-cine, University College London, London WCIE 611, U.K. Shraga F. Goldmann Red Cross Blood Bank Ulm, Department of
Trans-fusion Medicine, University of Ulm, D-7900 Ulm, F.R.G.
Walter Goldschmidts Pediatric Branch, Clinical Oncology Program, Di-vision of Cancer Treatment, National Cancer Institute, National In-stitutes of Health, Bethesda, Maryland 20205, U.S.A.
B. Grosbois Groupe Universitaire de Recherche en Immunologie Fon-damentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
Keith Guy Medical Research Council Clinical and Population Cytoge-netics Unit, Western General Hospital, Edinburgh, U.K.
Gunter J. Hammerling Institut fUr Immunologie und Genetik, Deutsches Krebsforschungszentrum, D-6900 Heidelberg, F.R.G. Kathleen R. Hagert University of Minnesota, Minneapolis, Minnesota
55455, U.S.A.
Wayne W. Hancock Nephrology Department, Prince Henry's Hospital, Melbourne, Australia
Toshiro Hara Cancer Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, U.S.A.
D.L. Hardie Department of Immunology, The Medical School, U niver-sity of Birmingham, Birmingham BI5 2TJ, U.K.
F. Herrmann Abt. Haematologie, Klinikum Steglitz, Freie Universitaet Berlin, Berlin, F.R.G.
xvi Contributors
R.D. Hockett, Jr. University of Minnesota, Minneapolis, Minnesota
55455, U.S.A.
Peter Hokland The University Department of Medicine and
Hematol-ogy, Aarhus Amtssygehus, Denmark DK-8000
Keizo Horibe Human Immunogenetics Laboratory, Memorial
Sloan-Kettering Cancer Center, New York, New York 10021, U.S.A.
J.C. Horowitz Division of Tumor Immunology, Dana-Farber Cancer
In-stitute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Donald R. Howard Terry Fox Laboratory, B.C. Cancer Research
Cen-tre, Vancouver, British Columbia, Canada
W. Hunstein Medizinische Universitats-Poliklinik, D-6900 Heidelberg,
F.R.G.
Yoshifumi Ishii Department of Pathology, Sapporo Medical College, S 1
WI7 Sapporo 060, Japan
G.D. Johnson Department of Immunology, University of Birmingham,
Medical School, Birmingham B15 2TJ, U.K.
D.B. Jones University Department of Pathology, General Hospital,
Southampton S09 4XY, U.K.
David G. Jose Immunogenetics Research Unit, Cancer Institute,
Mel-bourne, Australia 3000
Lawrence K.L. Jung Cancer Research Program, Oklahoma Medical
Re-search Foundation, Oklahoma City, Oklahoma 73104, U.S.A.
John H. Kersey University of Minnesota, Minneapolis, Minnesota
55455, U.S.A.
M. Khan Department of Immunology, University of Birmingham,
Medi-cal School, Birmingham B 15 2TJ, United Kingdom
S. Kiesel Medizinische U niversitats-Poliklinik, D-6900 Heidelberg,
F.R.G.
Kokichi Kikuchi Department of Pathology, Sapporo Medical College,
SI W17 Sapporo 060, Japan
James Kiwanuka Pediatric Branch, Clinical Oncology Program,
Divi-sion of Cancer Treatment, National Cancer Institute, National Insti-tutes of Health, Bethesda, Maryland 20205, U.S.A.
Jeroen Knops Institute fUr Immunologie und Genetik, Deutsches
Krebs-forschungszentrum, D-6900 Heidelberg, F.R.G.
Robert W. Knowles Human Immunogenetics Laboratory, Memorial
Sloan-Kettering Cancer Center, New York, New York 10021, U.S.A.
Yasuo Kokai Department of Pathology, Sapporo Medical College, S 1
WI7 Sapporo 060, Japan
Norbert Kraft Nephrology Department, Prince Henry's Hospital,
Mel-bourne, Australia
Bernhard Kubanek Red Cross Blood Bank Uim, Department of
Contributors XVlI
H. Ladyman Department of Haematology, John Radcliffe Hospital, Ox-ford OX3 9DU, U.K.
F. Lancelin Groupe Universitaire de Recherche en Immunologie Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
Gilles J. Lanzon Department of Pediatrics and the Tom Baker Can-cer Centre, University of Calgary, Calgary, Alberta, Canada T2N 4Nl
Thierry Lavabre-Bertrand Service des Maladies du Sang, C.H. U., Montpellier, France
A.M. Lebacq-Verheyden Unite de Recherches sur les Maladies du Sang, Universite Catholique de Louvain, Brussels, Belgium
Tucker W. LeBien Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 55455, U.S.A. R. Leblay Groupe Universitaire de Recherche en Immunologie
Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U. Rennes, France
Jeffrey A. Ledbetter Immunobiology Group, Genetic Systems Corpora-tion, Seattle, Washington 98121, U.S.A.
Grace T.H. Lee Immunogenetics Research Unit, Cancer Institute, Mel-bourne, Australia 3000
N.R. Ling Department of Immunology, University of Birmingham, Medical School, Birmingham B15 2TJ, U.K.
B. Michael Longenecker Department ofimmunology, University of Al-berta, Edmonton, AlAl-berta, Canada T6G 2H7
W.D. Ludwig Abt. Hamatologie, Klinikum Steglitz, Freie Universitat Berlin, Berlin, F.R.G.
I.C.M. MacLennan Department of Immunology, University of Birming-ham, Medical School, Birmingham B15 2TJ, U.K.
Ian T. Magratb Pediatric Branch, Clinical Oncology Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205, U.S.A.
Abby L. Maizel Department of Pathology, Section of Pathobiology, The University of Texas Cancer Center, Houston, Texas 77030, U.S.A.
Gerald E. Marti Hematology Service, Clinical Pathology Department, National Institutes of Health, Bethesda, Maryland 20205, U.S.A. M. Marty Groupe Universitaire de Recherche en Immunologie
Fonda-mentale et Appliquee, Service de Medecine Interne, Departement d'Hematologie, C.H.U., Rennes, France
D.Y. Mason Department of Haematology, John Radcliffe Hospital, Ox-ford OX3 9DU, U.K.
Robert T. McCormack Department of Laboratory Medicine/Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, U.S.A.
xviii Contributors
G. Merdrignac Groupe Universitaire de Recherche en Immunologie Fondamentale et Appliquee, Service de Medecine Interne, Departe-ment d'Hematologie, C.H.U., Rennes, France
Karin Meyer The University Department of Medicine and Hematology,
Aarhus Amtssygehus, Denmark DK-8000
Patricia M. Michael Immunogenetics Research Unit, Cancer Institute,
Melbourne, Australia 3000
Jordi Mila Servicio de Inmunologfa, Hospital Clinic i Provincial, 08036
Barcelona, Spain
Gerhard Moldenhauer Institut fUr Immunologie und Genetik, Deutsches
Krebsforschungszentrum, D-6900 Heidelberg, F.R.G.
Frank Momburg Institut fur Immunologie und Genetik, Deutsches
Krebsforschungszentrum, D-6900 Heidelberg, F.R.G.
John Morgan Department of Pathology, Section of Pathobiology, The
University of Texas Cancer Center, Houston, Texas 77030, U.S.A.
Marion Moxley Medical Research Council, Clinical and Population
Cy-togenetics Unit, Western General Hospital, Edinburgh, U.K.
Lee M. Nadler Division of Tumor Immunology, Dana-Farber Cancer
Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
P .D. Nathan Department of Immunology, University of Birmingham,
Medical School, Birmingham B15 2TJ, U.K.
Leonard M. Neckers Laboratory of Pathology, National Cancer
Insti-tute, National Institutes of Health, Bethesda, Maryland 20205, U.S.A.
G. Pallesen University Institute of Pathology, Kommunehospitalet,
DK-8000 Aarhus C, Denmark
Edward Park Division of Tumor Immunology, Dana-Farber Cancer
In-stitute, Boston, Massachusetts 02115, U.S.A.
Carlo Parravicini Fifth Department of Pathology, University of Milan,
Milan, Italy
Antonio Pezzutto Medizinische Universitats-Poliklinik, D-6900
Heidel-berg, F.R.G.
Glenn R. Pilkington Immunogenetics Research Unit, Cancer Institute,
Melbourne, Australia 3000
Samuel J. Pirruccello Department of Laboratory Medicine/Pathology,
University of Minnesota Medical School, Minneapolis, Minnesota 55455, U.S.A.
Stefania Pittaluga Laboratory of Pathology, National Cancer Institute,
National Institutes of Health, Bethesda, Maryland 20205, U.S.A.
Jeffrey L. Platt Department of Laboratory Medicine/Pathology,
Uni-versity of Minnesota Medical School, Minneapolis, Minnesota 55455, U.S.A.
Philippe Poncelet Immunotoxin Project, Research Center Clin-Midy,
Contributors xix
Anand Raghavachar Red Cross Blood Bank Ulm, Department of Trans-fusion Medicine, University of Ulm, D-7900 Ulm, F.R.G.
A.M. Ravoet Unite de Recherches sur les Maladies du Sang, Universite Catholique de Louvain, Brussels, Belgium
Frances Rawle ICRF Human Tumor Immunology Group, School of Medicine, University College London, London 'YClE 6JJ, U.K. H. Riehm Abt. Padiatrie VI, Medizinische Hochschule Hannover,
Han-nover, F.R.G.
John T. Sandlund Pediatric Branch, Clinical Oncology Program, Divi-sion of Cancer Treatment, National Cancer Institute, National Insti-tutes of Health, Bethesda, Maryland 20205, U.S.A.
Volker Schirrmacher Institute of Immunology and Genetics, German Cancer Research Center, D-6900 Heidelberg, F.R.G.
Stuart F. Schlossman Division of Tumor Immunology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, U.S.A.
Reinhard Schwartz Institut fUr Immunologie und Genetik, Deutsches Krebsforschungszentrum, D-6900 Heidelberg, F.R.G.
Geraldine Shu Immunobiology Group, Genetic Systems Corporation, Seattle, Washington 98121, U.S.A.
Bruce Slaughenhoupt Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, U.S.A.
C.M. Steel Medical Research Council Clinical and Population Cytoge-netics Unit, Western General Hospital, Edinburgh, U.K.
Tsuyoshi Takami Department of Pathology, Sapporo Medical College, SI W17 Sapporo 060, Japan
Fumio Takei Terry Fox Laboratory, B.C. Cancer Research Centre, Vancouver, British Columbia, Canada
Takashi Takei Department of Pathology, Sapporo Medical College, S 1 W17 Sapporo 060, Japan
Thomas F. Tedder Cellular Immunobiology Unit, Tumor Institute, Uni-versity of Alabama in Birmingham, Birmingham, Alabama 35294, U.S.A.
E. Thiel Institute fUr Haematologie, Miinchen, F.R.G.
David A. Thorley-Lawson Departments of Pathology and Medicine, . Tufts University School of Medicine, Boston, Massachusetts 02115, U.S.A.
Jane B. Trepel Laboratory of Pathology, National Cancer Institute, Na-tional Institutes of Health, Bethesda, Maryland 20205, U.S.A. Ramon Vilella Servicio de Imnmunologfa, Hospital Clinic i Provincial,
08036 Barcelona, Spain
Jordi Vives Servicio de Inmunologfa, Hospital Clinic i Provincial, 08036 Barcelona, Spain
L. Walker Department of Immunology, University of Birmingham, Medical School, Birmingham B15 2TJ, U.K.
xx Contributors
Janis J. Weis Department of Medicine, Harvard Medical School,
De-partment of Rheumatology & Immunology, Brigham and Women's Hospital, Boston, Massachusetts 02115, U.S.A.
P. Wernet Medizinische Universitats-Klinik, Tiibingen, F.R.G.
James G. White University of Minnesota, Minneapolis, Minnesota
55455, U.S.A.
D.H. Wright University Department of Pathology, General Hospital,
Southampton S09 4XY, U.K.
Hiroo Yuasa Department of Pathology, Sapporo Medical College, SI
WI7 Sapporo 060, Japan
Theodore F. Zipf Faculty of Medicine, University of Calgary, Calgary,
Alberta, Canada T2N 4N 1
Heddy Zola Flinders Medical Centre and Flinders University of South