Grow it. Read it. See it. Count it.
Cytation™3 combines automated digital widefield microscopy and conventional multi-mode microplate detection in a unique patent pending system
designed to capture rich phenotypic information and collect meaningful quantitative data in a single platform. Incorporating a powerful fluorescence and brightfield imaging module with the patented Hybrid Technology™ for multi-mode detection provides a streamlined workflow for cell-based and biochemical assays without the complexity and expense that is typical of automated microscopes and multi-mode detection systems. The modular architecture allows users to customize Cytation3 to suit their needs now…and upgrade when assay and application requirements change in the future.
Cytation3
Cell Imaging Multi-Mode Reader
Automated Microscopy & Multi-Mode Detection
The image on the reverse side is BPAEC cells from FluoCells® prepared slide #2, captured on Cytation3 at 4x magnification. FluoCells is a registered trademark of Life Technologies™, Inc.
Cytation3
Cell Imaging Multi-Mode Reader
Automated Microscopy & Multi-Mode Detection
Fully Automated Imaging System
Auto X Y stage simplifies precise sample positioning and stage movement
Auto LED controls up to 100% intensity output Auto exposure applies an average exposure setting across the plate
Auto focus determines the plane of focus automatically based on plate or sample vessel dimensions
Multi-Mode Microplate Detection
The microplate reader in Cytation3 measures fluorescence intensity, time resolved
fluorescence and fluorescence polarization along with luminescence and UV-Vis
absorbance. The patented Hybrid Technology brings filter-based and monochromator-based optics together to provide the powerful detection capabilities required for cell-based and other biochemical assays. Multiple parallel detectors speed the measuring time, and the dedicated filter-based optics are optimized for live cell assays.
Modular & Upgradable
Cytation3 has a modular architecture, so it’s easy to select any combination of the independent systems now, and upgrade as the laboratory’s needs change.
Monochromator optics Filter optics
Grow It
Cytation3 can be outfitted with a CO2 / O2 control module to provide the
optimal environment for cell growth, along with programmable temperature control to 45 ºC, plus orbital and linear shaking. An available dual reagent dispenser is a valuable tool for precise reagent dispensing - ideal for dispense/read operations.
Count It
Cytation3 is controlled by Gen5 software for quantitative and image data collection and
analysis. Cell counting in microplates is as easy as clicking here and there. Cell counts and image statistics are automated calculated, and analysis options are intuitive and powerful, whether the samples are in microplates or slides.
Read It
Plate measurement options are nearly limitless in Cytation3. The multi-mode microplate
detection system offers fluorescence intensity, time-resolved fluorescence, fluorescence polarization, UV-Vis absorbance, flash and glow luminescence. All modes are available for measuring samples in 6- to 384-well plates to suit a wide range of applications. If cell imaging is the next step in your workflow, Gen5™ offers
a hit-picking function to select just the wells whose intensity meets the imaging threshold. Saves time and greatly reduces data analysis and storage requirements.
See It
There’s never been an easier or faster way to image your cells than in Cytation3! The versatile
imaging system provides up to four fluorescent color channels plus brightfield imaging, making it the ultimate benchtop imaging workstation. Gen5 software offers Auto Easy functions for all steps of imaging, from managing focus to LED intensity for users at all levels (manual operation is also available). Excellent image quality is assured with the 16 bit grayscale CCD camera with Sony chip, along with other high quality optical components.
Image Analysis Made Easy
Gen5™ software automates the entire
imaging process and makes cellular image analysis simple and intuitive. Cell counting and sub-population data are calculated as images are acquired, and Gen5’s data analysis tools are easily applied. It’s powerful software for imaging and microplate analysis, but is designed for users of all skill levels, including those without prior imaging experience. Captured images are analyzed, optimized, displayed and quickly and output in a variety of formats for printing, exporting and saving. Gen5 can import.tif files from other imaging software as well, for added convenience.
NIH3T3 cells 20x
NIH3T3 cells 20x (zoomed)
It takes only a few minutes, not hours, to count cell nuclei in all the wells of a microplate. Imagine the improved workflow and time savings Cytation3 and Gen5 can offer, especially when testing multiple conditions and monitoring cell populations in microplates.
Purposeful Image Statistics
General statistics are calculated on the image’s pixel values, providing relevant information like average pixel intensity and standard deviation. Show correlation between methods, confirm a hit, calculate the dose response or analyze migration behavior, all within the automated or manual Image Statistics tools in Gen5.
Cell Counting in Minutes
Zebrafish-4x
Sub-Population Analysis
When multiple cell sub-populations are present in a sample, Gen5 can sort by intensity or morphology, thus enabling analysis of transfection efficiency, nuclear translocation or cell cycle assays.
Multi-Mode Detection Data Analysis
Use the intuitive Gen5 interface to control Cytation3, collect data and analyze the data with the powerful tools available for qualitative and quantitative assays. Kinetic analysis, powerful curve-fitting options, EC50 and potency calculations are among the many tools available in the software to get the results you need – fast!
Efficient Workflow
Whether the assay is conducted in microplate, slide, petri dish or in the low-volume Take3™ plate, Cytation3 and Gen5 simplify the workflow by offering
multi-mode detection, imaging and analysis in one platform. Protocols for measuring endpoint or kinetics, or imaging an entire well or montage in a well, along with many other assay steps, are easily created in the Gen5 procedure steps for efficiency, speed and optimal assay performance.
Universal Image Format
Gen5 images are compatible with many image analysis software programs including Image J or Cell Profiler for custom analysis. Raw data is saved in native grayscale .tif format, while presentation images may be saved in color in .tif, .jpg, .png, .gif, .bmp and .emf formats.
Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader to Monitor IL-6 Secretion using HTRF detection and Cell Viability Using Digital Widefield Fluorescence Microscopy
SKOV-3 cells; blue is DAPI-stained nuclei, green is Phalloidin actin staining.
U-2 OS cells stained for mitochondria, F-actin and nucleus.
Imaging of BacMam Transfected Human U-2 OS Cells.
Use of Gen5 software’s “Split touching objects” on cell counting. Scale bar indicates 10 μm. Automated Tissue Culture Cell Fixation
and Staining in Microplates
Using the EL406™ Combination Washer Dispenser to Wash, Fix, Permeabilize, Stain and Add Antibody
Imaging of BacMam Transfected Human U-2 OS Cells in Microplates
Optimization of Tranfection Conditions Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader and Gen5 Data Analysis Software
Analysis of Nuclear Stained Cells
Using the Cytation3 Cell Imaging Multi-Mode Microplate Reader with DAPI-stained Cells
One Instrument.
Many Applications.
Cytation3 combines digital widefield microscopy and multi-mode detection for a more complete picture than either separate technology. This unique combination offers quantitative and phenotypic rich qualitative data in parallel, and when combined with specialized, optional accessories, the range of biochemical, live cell and fixed cell applications is almost endless.
Cell imaging, analysis, proliferation and toxicity
Cell counting Biomarker quantification Drug discovery Genetic analysis
Drug absorption and metabolism Environmental testing Food safety
Nucleic acid and protein quantification
For more applications, visit www.cytation3.com
Specifications
General
Imaging mode Fluorescence and brightfield
Detection mode Monochromators: FL, Lum, UV-Vis Abs Filters: FL, TRF, FP, Lum
Read method Endpoint, time-lapse, kinetic, well mode, montage
Labware type 6- to 384- well microplates, microscope slides, cell culture flasks (T25), Compatible with Take3™
Micro-Volume Plates with 2 µL microspots
Temperature
control To 45 °C +0.5 °C at 37 °C Shaking Linear, orbital, double orbital
Automation BioStack and 3rd party automation compatible
CO2 and O2
control 0 - 20% COwith optional Gas Controller2 control and 1 - 19% O2 control,
Software Gen5™ Data Analysis Software included
Imaging System
Camera 16-bit gray scale, Sony CCD, 1.1 megapixel
Imaging filter
cube capacity 4 onboard, user-replaceable filter cubes Imaging filter
cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5 and CY7
Imaging LED
cubes available 365 nm, 465 nm, 590 nm, 523 nm, 505 nm, 623 nm, 740 nm
Objective
capacity 2 onboard user-replaceable objectives Available
objectives 2x, 2.5x, 4x, 10x, 20x, 40x, 60x Image collection
rate 96 wells, 1 color (DAPI), 4x, 6 minutes 96 wells, 3 colors, 4x, 12 minutes
Fluorescence Intensity
Sensitivity Monochromators:
Top: fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Bottom: fluorescein 5 pM typical (0.5 fmol/well 384-well plate)
Filters/mirrors:
Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Light source Xenon flash lamp
Wavelength
selection Double grating monochromators (top/bottom) and, Deep blocking bandpass filters/dichroic mirrors (top)
Wavelength
range Monochromators: 250 - 700 nm Filters: 200 - 700 nm (850 option)
Dynamic range 5 decades
Detection
system Two PMT detectors: one for monochromator system, one for filter system
Luminescence
Sensitivity Monochromator system: 20 amol ATP typical (flash)
Filter system: 10 amol ATP typical (flash)
Wavelength
range 300 - 700 nm Dynamic range ≥6 decades
Fluorescence Polarization
Sensitivity 5 mP at 1 nM fluorescein typical
Wavelength
range 320 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Sensitivity Europium 100 fM typical with filters (10 amol/ well in 384-well plate)
Europium 1200 fM typical with monos (120 amol/well in 384-well plate)
Light source Xenon flash lamp
Wavelength
range Monochromators: 250 - 700 nm Filters: 200 - 700 nm (850 option) Absorbance
Light source Xenon flash lamp
Wavelength selection Monochromator Wavelength range 230 - 999 nm, 1 nm increment Bandpass 4 nm (230-285 nm), 8 nm (>285 nm) Dynamic range 0 - 4.0 OD Resolution 0.0001 OD Reagent Dispensers
Number 2 syringe pumps
Dispense
volume 5 - 1000 µL in 1 µL increment Dead volume 1 mL, 100 µL with back flush
Plate geometry 6- to 384-well microplates
Dispense
precision <2% at 50-200 µL Dispense
accuracy ±1 µL or 2%
Physical Characteristics
Power 130 Watts max.
Dimensions 20” D x 16.5” W x 17.5” H (50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)
Regulatory CE and TUV marked. Models for In Vitro Diagnostic use are available.
Specifications are subject to change.
Dual Reagent Dispenser
Gas Controller Module
Cytation3 and BioStack Microplate Stacker
High Quality Imaging.
High Quality Components.
Cytation3 unites BioTek’s microplate instrumentation expertise with components from leading imaging experts. This combination provides unsurpassed workflow efficiency and a truly unique live cell assay solution.
Olympus and Zeiss Objectives LED Cubes and Filter Cubes with Semrock Filters
At BioTek, our philosophy transcends conventional thinking and challenges the old ways. We develop fresh, original solutions by unifying concepts that often appear to be opposed. It means to shape and reshape. To engineer, build, deliver and support products that best serve the marketplace by providing what they need, when they need it.
Think Possible. It’s the difference
between leading and following.
BioTek Instruments, Inc.
Tel: 802-655-4040 • Toll-Free: 888-451-5171 • Outside the USA: 802-655-4740 www.biotek.com
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In Canada:
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