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Genome engineering for improved recombinant protein expression in Escherichia coli

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Figure

Figure 1 Metabolic engineering strategies to enhance the flux through a pathway. The various strategies used to improve the flux from acellular intermediate to the desired product is shown
Figure 3 Cellular factors controlling the rate of translation. These factors which directly effect translation efficiency are a) mRNA RBS secondarystructure affecting the Translation Initiation Rates (TIR) and the UTR sequences, b) Degradation of ribosomes
Figure 4 Various pathways for protein translocation (Type I and II). The type II Sec-dependent pathway is the most commonly used andgets overloaded leading to accumation of mis folded proteins in the cytoplasm
Figure 5 Overall Strategy for host cell engineering using rational and high throughput methods

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