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Crosstalk between chromatin structure, cohesin activity and transcription

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Fig. 1 Genome‑wide nucleosome profiles in cohesin and histone deposition mutants. a Representative nucleosome profile by MNase‑seq of wild type, scc1‑73, t::HHF2 and t::HHF2 scc1‑73 cells synchronized in G1 and released until metaphase in nocodazole‑contai
Table 1 Number of  altered nucleosomes (in position, fuzziness and/or  occupancy) upon  histone depletion (in t::HHF2 cells) or  cohesin inactivation (in scc1-73 cells) as  compared to  the  profile of  nucleosomes in  wild-type cells
Fig. 2 Transcription downregulation in promoters that contain nucleosomes with higher occupancy (up‑nucleosomes), in < 0.01 are shown in green for downregulated (445) and in red for upregulated (569)
Fig. 4 Cohesins are not involved in the deposition of newly synthesized histones. ChIP analysis of the incorporation of newly synthesized histone H3 (acetylated in lysine 56) in wild‑type and scc1‑73 cells that have been synchronized in G1 and released int
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