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Knockdown of subunit 3 of the COP9 signalosome inhibits C2C12 myoblast differentiation via NF-KappaB signaling pathway

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Academic year: 2020

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Figure

Fig. 1 Down regulation of CSN3 in C2C12 cell lines.blot is shown from samples separated on a single gel.passage C2C12 were infected with lentiviral vectors expressing shCSN3-Med, shCSN3-Low or non-target shRNA (shNT)
Fig. 2 Knockdown of CSN3 decreases the protein levels of other CSN subunits a Proteins were extracted from proliferating shNT-control, shCSN3-Medor shCSN3-low stable cells lines
Fig. 3 Effect of CSN3 Knockdown on myoblast fusion. (a, b, c) Phase contrast images (20X objective) of stable cell lines grown in differentiation(DM) media from 0 to 9 days
Fig. 4 Effect of CSN3 knockdown on the expression of myogenic markers. a Representative immunoblots of myogenin and MHC expression inC2C12 cell lines from samples run on a single gel
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