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Construction of an immunotoxin via site-specific conjugation of anti-Her2 IgG and engineered Pseudomonas exotoxin A

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Academic year: 2020

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Figure

Fig. 1 (a) Chemical structure of azidophenylalanine (AzF). b Amino acid sequence of the PE24-TAG construct
Fig. 2 Reduction and reoxidation of trastuzumab variants. Reduced or reoxidized IgGs were analyzed by SDS-PAGE under a non-reducingcondition
Fig. 3 Conjugation of trastuzumab variants and PE24-AzF. Analysis of SDS-PAGE was done under a non-reducing condition for reduced,reoxidized, and PE24-conjugated trastuzumab
Fig. 5 (a) ADP-ribosylation of eEF-2. Wheat germ extract including eEF-2 was incubated with biotinylated NAD+ in the presence of PE24-AzF ortrastuzumab-PE24, and then the reactions were analyzed by western blot using streptavidin-HRP conjugate
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