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Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway.

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Academic year: 2020

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Figure

Figure 3. Molecular analysis of gene transfer efficiency in male and femalemice. (A) Southern blot analysis of undigested low-molecular-weight Hirt DNAextracted from liver 3 days (3 d), 10 days (10 d), and 20 days (20 d) after portal veininfusion of vector
Figure 5. Effect of androgens on rAAV-mediated transgene expression.(A) Human FIX expression in C57Bl/6 competent mice 6 weeks after tail veinadministration (Mtogether with the standard error of the mean of each cohort containing between 3 to 7mice
Figure 6. Effects of androgens on rAAV transduction and attachment to humanHuH7 cell line.density of 1prior to gene transfer
Figure 7. Competitive gel mobility shift assay. (A) Schematic of the AAV-2 ITR demonstrating the positions of the Rep-binding site as well as the pertinent 30 mericexcess of unlabeled cognate

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