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Functional Pentameric Formation via Coexpression of the Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Fusion Protein Subunit with a Neutralizing Epitope of ApxIIA Exotoxin Improves the Mucosal Immunogenicity and Protection against Challenge b

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Figure

FIG. 1. (A) Schematic diagram of pYEGPD-TER and pYIGPD-TER yeast expression vectors. The boxes represent genes or their corre-sponding functional domains
TABLE 1. Plasmids used in this study
FIG. 2. Western blot analysis of recombinant LTB, ApxIIA#5, andLTB::ApxIIA#5 in CFE. The anti-LTB (A and B) and anti-ApxIIA (C
FIG. 3. GM1 ganglioside binding assay (A and B) and quantitative ELISA (C and D) of yeast-expressed LTB and LTB::ApxIIA#5
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