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Investigation of Interfacial Interactions in Surfactant-mediated Shear Protection of Animal Cell Culture.

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Figure 1.1: Summary of interfacial interactions in a fed-batch bioreactor process. Bubble bursting, foam entrapment, and turbulence from agitation and gas entrance velocity can lead to cell damage
Figure 1.2: Example of cell damage during sparging adapted from Ref. [11]. Maximum cell growth is achieved in batch 1 with no sparging
Figure 1.3: Estimated characteristic strength of several biological particles [14]. The strength of mammalian cells is lowest, indicating their fragility relative to other cell types
Figure 1.4: Sequence of events during bubble collapse. During initial film breakage (0 – 0.57 ms), the film recedes to a point
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Effect of surfactant on rotational and lateral membrane fluidity Discussion Foam stability and cell aggregation experiments Poloxamer concentration in the medium Bioreactor studies with varied shear environment Impact and future outlook

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