Sex-Induced Silencing Operates During Opposite-Sex and Unisexual Reproduction in Cryptococcus neoformans
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![Figure S3. Wild‐type serotype A SXI2a gene complements mating defects of a serotype D sxi2aΔ mutant strain. A wild‐type serotype D α strain (JEC21α) was cocultured with three mating partners [wild type serotype D a strain (JEC20a), a sxi2aΔ mutant strain (CHY768 ura5) in the JEC20a background, or the sxi2aΔ mutant strain in which the wild type serotype A allele of SXI2a was introduced by transformation (XW219)] on V8 agar medium for 3 days in the dark at 22°C to examine the production of hyphae during mating. Inspection of the wild‐type and complemented mating reactions at day=7 revealed basidia and spore chains (data not shown).](https://thumb-us.123doks.com/thumbv2/123dok_us/1556027.1191061/16.612.98.517.220.342/complements-cocultured-background-introduced-transformation-production-inspection-complemented.webp)
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