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Distribution of TNF alpha like proteins correlates with some regions of programmed cell death in the chick embryo

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Figure

Fig. 2. Sectionsthrough11mthe nervecord. (a)Dav 3 (stage18), Sectionthroughthe neuraltube (nO and norochord(n) at the level of thewing budsTUNEL-positlve cells are presentInthe floor plate of the neural tube (arrowl
Fig. 9. Doublelabeledthesectionthroughheart!day6,stage29!. TNFrtinlnwnoreactlvityis seenprimafllyin thedevelopingcardiacmuscleoftheventri-clewall(darkblue)whileTUNEL-posirivecells(red)are also seenin thistissue.Bar,SOJlnl
Fig. 11. TheL929-8TNF(lbioassay.!aJ The effectof day 3 (stage 18) chickembryohomogenareat a final dilution of1:36 on the opricaldensityofL929-8 cells alone (H) or In the presenceof neutralizingantibodiesto TNFa(Ab1, Genzymeand Ab2, GenenrechJ.The optical density/3 slgmflcantlyreduced(implyingIncreasedcelldeath)in the presenceof embryohomogenatecomparedto rhatwirh embryo homogenatein the presenceof TNFa-specificneutralizingantibodies(p<O.005, Ab 1and p<O.005, Ab2J(bl The percentviabilityof L929-8cellschallengedwithexo;;enousTNFaat concentratIOnsof 0 01 pg/mland 5 pg/mlor withembryohomogenateat a fmaldilutionof 1:36 alone (H) or embryo homogenatein the presenceof neutralizingantibodiesto TNFa (Abl,Genzymeand Ab2,Genentech).

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