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Expression of neurotrophin 4 mRNA during oogenesis in Xenopus laevis

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Figure

Fig. 1. NT-4 mRNApm thickXenopuscontent.and the absenceat large (stageexpressionin theXenopuslaevisovary.Ovary from adult Xenopus laevis was sectionedIn a cryostat(14 sections) and the sectionswere then hybfldlzedto theIndicated53-meroligonucleotideslabeledwithJ~S-dA TP usingterminaldeoxynucleotldyltransferase.(A) HybridizaMnuSing aNT-4 mRNAspecificprobe.(S) Hybridizationusing acontrolchickenBDNFmRNAoligonucleotideof sImilarG+CAfter hybridization,sectionswere washedIn lxSSC at55"C followedby e>o.pasure to X-ray film for 10 days, ShownInthe figure are photographsof the developedX-ray films.Notethe intenselabelingover many small cells with the NT-4 probeof labelingwith the control probe, Arrowspoint VI) oocyteswhichare not labeledwitheitherofthe two probes.Scale bar, 2 mm.
Fig. 3. levelsof NT.4 mANAXenopusgrains overain oocytesat differentstagesof oogenesis.Emulsionautoradiographs(shownin Fig

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