BIOCHEMISTRY DEPARTMENT

(1)

BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

ALBUMIN 33 – 46 g/L

ALK–PHOSPHATASE Up to 11 months Male 133 – 426 U/L

Female 196 – 553 U/L

11+ months – 2 years Male 133 – 287 U/L

2+ – 8 years Male 133 – 287 U/L

8+ – 11 years Male 133 – 340 U/L

11+ – 15 years Male 133 – 468 U/L

15+ – 17 years Male 60 – 340 U/L

17+ – 18 years Male 60 – 162 U/L

18+ – 19 years Male 60 – 162 U/L

Adult 30 – 110 U/L

ALPHA–1 ANTITRYPSIN 0.88 – 1.74 g/L

ALT (SGPT) 4 – 43 U/L

AMYLASE 27 – 110 U/L

AMYLASE URINE Random up to 350 U/L

Per 24 hours up to 440 U/d

ANDROSTENEDIONE Male 2.1 – 10.8 nmol/L

Female 1.2 – 13.7 nmol/L

ANION GAP with K⁺ 10 – 20 mmol/L

no K⁺ 10 – 16 mmol/L

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

ANTI DNA Negative < 20 IU/mL

Borderline 20 – 25 IU/mL

Positive > 25 IU/mL

AST (SGOT) 6 – 42 U/L

ASO less than 116 KU/L

BILIRUBIN TOTAL Greater than 5 days 3 – 18 mol/L

2 – 5 days 68 – 200 mol/L

Less than – 2 days 100 – 120 mol/L

BILIRUBIN DIRECT 0 – 5 mol/L

CALCIUM Greater than 18 years 2.15 – 2.60 mmol/L

11 days – 18 years 2.20 – 2.70 mmol/L

Up to 10 days 1.90 – 2.60 mmol/L

CALCIUM URINE Random 1.7 – 5.0 mmol/L

24–Hours 1.2 – 6.2 mmol/d

CARBAMAZEPINE 17 – 51 mol/L

CA125 0 - 35 U/mL

CERULOPLASMIN 200 – 640 mg/L

CHLORIDE 95 – 108 mmol/L

CHLORIDE URINE Random 15 – 250 mmol/L

24–Hour 110 – 250 mmol/d

CHOLESTEROL Less than 29 years Target less than 4.60 mmol/L Greater than 29 years Target less than 5.20 mmol/L

CHOLESTEROL/HDL RATIO If Risk Target

Low Risk less than 6.0

Moderate Risk less than 5.0

High Risk less than 4.0

CK Male less than 210 U/L

Female less than 170 U/L

CO2 21 – 31 mmol/L

COMPLEMENT C3 0.70 – 1.76 g/L

COMPLEMENT C4 0.16 – 0.45 g/L

hs CRP Low Risk less than 1.0 mg/L

Average Risk 1.0 – 3.0 mg/L

High Risk greater than 3.0 mg/L

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

CORTISOL A.M. 185 – 624 nmol/L

P.M. < 280 nmol/L

Random

Cannot be determined

Free, Urine 24–Hour 138 – 524 nmol/d

Total, Urine 24–Hour 440 – 1800 nmol/d

C–PEPTIDE 298 – 2350 pmol/L

CREATININE Male (greater 10 years) 60 – 127 mol/L Female (greater 10 years) 60 – 115 mol/L

5 – 10 years 44 – 71 mol/L

Less than 5 years less than 44 mol/L

CREATININE URINE Random Male 5.0 – 13.0 mmol/L

Random Female 3.0 – 11.0 mmol/L

24–Hour Male 7.0 – 20.0 mmol/d

24–Hour Female 4.0 – 16.0 mmol/d

CREATININE CLEARANCE 1.25 – 1.92 mL/s

DHEA–S Male less than 14.1 mol/L

Female less than 11.0 mol/L

DIGOXIN 0.6 – 2.6 nmol/L

DILANTIN (PHENYTOIN) 40 – 80 mol/L

ESTRADIOL Non Pregnant Female:

Mid Follicular 99 – 448 pmol/L Mid–Cycle Peak 350 – 1590 pmol/L Mid Luteal 180 – 1068 pmol/L Post Menopausal 0 – 147 pmol/L

Male 0 – 172 pmol/L

ETHYL ALCOHOL None Detected

FERRITIN Male 25 – 200 g/L

Female 11 – 145 g/L

Newborn 5 – 100 g/L

FOLATE ERCS

(RBC FOLATE) greater than 829 nmol/L

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

FSH Female:

Mid Follicular 3.9 – 8.8 IU/L Mid–Cycle Peak 4.5 – 22.5 IU/L Mid Luteal 1.8 – 5.1 IU/L Post Menopausal 16.7 – 113.6 IU/L

Male 1.3 – 19.3 IU/L

GAMMA GT (GGT) Male 5 – 50 U/L

Female 5 – 40 U/L

GASTRIN 13 – 115 ng/L

GLUCOSE FASTING New Born – 3 years 2.5 – 5.0 mmol/L Children (3 – 12 years) 2.8 – 6.1 mmol/L Adolescent (13 – 18 years) 3.3 – 6.1 mmol/L

19 years – Adult 3.3 – 6.0 mmol/L

GLUCOSE RANDOM 3.3 – 7.8 mmol/L

GLUCOSE PC 2–Hour 3.30 – 7.80 mmol/L

GLUCOSE CHALLENGE Fasting 75 g Load 3.3 – 6.0 mmol/L

2–Hour 3.3 – 7.7 mmol/L

GLUCOSE CHALLENGE 50 G LOAD (GESTATIONAL)

(GESTATIONAL SCREEN) 1–Hour 3.3 – 7.8 mmol/L

GLUCOSE TOLERANCE TEST 75 G LOAD (GESTATIONAL)

(GESTATIONAL) FBS 3.3 – 5.3 mmol/L

1–Hour 3.3 – 10.6 mmol/L

2–Hour 3.3 – 8.9 mmol/L

100 G LOAD (GESTATIONAL)

FBS (> 18 years) 3.3 – 6.1 mmol/L

FBS (up to 18 years) 2.8 – 6.1 mmol/L

1–Hour 3.3 – 10.6 mmol/L

2–Hour 3.3 – 9.2 mmol/L

3–Hour 3.3 – 8.1 mmol/L

GLYCOSYLATED

HAEMOGLOBIN (HbA1C) 0.040 – 0.060

GROWTH HORMONE Male 0 – 1.0 g/L

Female 0 – 4.0 g/L

HAPTOGLOBIN 0.40 – 1.90 g/L

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

hCG Female, Non Pregnant less than 5 IU/L

Female, Pregnant:

1st week 5 – 50 IU/L

2nd week 40 – 300 IU/L

3rd week 100 – 1000 IU/L 1st trimester 10000 – 200,000 IU/L

(PEAK)

2nd trimester 3000 – 50,000 IU/L 3rd trimester 1000 – 50,000 IU/L

Male less than 2 IU/L

HDL Target greater than 1.29 mmol/L

HOMOCYSTEINE 5.0 – 15.4 mol/L

H. PYLORI Negative less than 15 U/mL

Borderline Positive 15 – 30 U/mL

Positive > 30 U/mL

HEPATITIS Negative

HIGH SENSITIVITY CRP Low Risk less than 1.0 mg/L

(hs CRP) Average Risk 1.0 – 3.0 mg/L

High Risk greater than 3.0 mg/L

IMMUNOGLOBULINS Greater than 16 Years:

IgG 5.64 – 17.65 g/L IgA 0.85 – 3.85 g/L IgM 0.45 – 2.50 g/L

IgE 0 – 432 g/L

PEDIATRIC IMMUNOGLOBULINS

Age IgG (g/L) IgA (g/L) IgM (g/L) 0 – 6 months 1.50 – 4.70 0.20 – 1.30 0.30 – 0.60 6 mos – 1 year 1.40 – 10.30 0.20 – 1.30 0.30 – 1.60 1 – 2 years 2.80 – 9.60 0.20 – 1.10 0.30 – 1.50 2 – 5 years 3.70 – 15.00 0.30 – 2.00 0.20 – 2.20 5 – 10 years 4.40 – 15.50 0.50 – 2.30 0.30 – 1.70 10 – 16 years 4.50 – 14.40 0.40 – 2.40 0.40 – 2.00

INSULIN Fasting 13 – 161 pmol/L

2-hour (post challenge)

Cannot be determined

Random

Cannot be determined

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

IONIZED CALCIUM 1.15 – 1.35 mmol/L

IRON 10 – 26 mol/L

IRON SATURATION 0.25 – 0.50

LD 76 – 200 U/L

LDL If clinically determined risk is: Target for LDL-C Low reduce by 50%

Moderate or High < 2.0 or reduce by 50 %

LH Female:

Mid Follicular 2.1 – 10.9 IU/L Mid Cycle 19.2 – 103.0 IU/L Mid Luteal 1.2 – 12.9 IU/L Post Menopausal 10.9 – 58.6 IU/L

Male 1.2 – 8.6 IU/L

LITHIUM 0.6 – 1.2 mmol/L

May reflect acute toxicity ≥ 1.5 mmol/L

LEAD BLOOD Up to 16 years up to 0.48 mol/L

Male (17 yrs and above) up to 1.00 mol/L

Female (17 – 45 yrs) up to 0.48 mol/L

Female (> 45 yrs) up to 1.00 mol/L

LIPASE 13 – 80 U/L

MAGNESIUM 0.65 – 1.05 mmol/L

MICROALBUMIN Random less than 15 mg/L

24–Hour less than 21 mg/d

MICROALBUMIN/ Male less than 2.0

CREATININE RATIO Female less than 2.8

PHENOBARBITAL 65 – 172 mol/L

PHOSPHATE 0.80 – 1.52 mmol/L

PHOSPHATE URINE Random 8.7 – 28.0 mmol/L

24–Hour 13 – 42 mmol/L

POTASSIUM 3.5 – 5.2 mmol/L

POTASSIUM URINE Random 17 – 80 mmol/L

24–Hour 25 – 120 mmol/d

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

PROGESTERONE Male 0.3 – 2.7 nmol/L

Female, Non Pregnant:

Mid Follicular 1.0 – 4.8 nmol/L Mid Luteal 16.4 – 59.0 nmol/L Post Menopausal < 0.3 – 2.5 nmol/L Female, Pregnant:

First Trimester 15.0 – 161.4 nmol/L Second Trimester 61.7 – 144.0 nmol/L

PROLACTIN Male 2.6 – 13.1 g/L

Female 3.3 – 26.7 g/L

PROTEIN SYNOVIAL FLUID 10 – 30 g/L

PROTEIN TOTAL 60 – 82 g/L

PROTEIN URINE RANDOM 0 – 0.15 g/L

PROTEIN URINE 24 HOUR 0 – 0.2 g/d

PSA TOTAL less than 4.00 g/L

PSA FREE less than 0.934

For Total PSA between 4 and 10 g/L, a Free/Total PSA ratio of < 0.25 is associated with an increased risk of prostate cancer.

PTH 1.3 – 8.2 pmol/L

RHEUMATOID FACTOR (RF) less than 20 IU/mL

RUBELLA Non–Immune less than 5 IU/mL

Inconclusive 5 – 10 IU/mL

Immune greater than 10 IU/mL

SODIUM 135 – 146 mmol/L

SODIUM URINE Random 20 – 230 mmol/L

24–hour 40 – 220 mmol/d

T3 FREE 2.9 – 6.0 pmol/L

T3 TOTAL 1.3 2.5 nmol/L

T4 FREE 7.2 – 21.0 pmol/L

TESTOSTERONE, TOTAL Female 0.4 – 2.6 nmol/L

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BIOCHEMISTRY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS

Male 6.0 – 27.0 nmol/L

TESTOSTERONE, FREE Male 15.6 – 146 pmol/L

Female 0 – 14.2 pmol/L

THEOPHYLLINE 55 – 110 mol/L

THYROGLOBULIN < 45 pmol/L

TIBC 45 –63 mol/L

TRIGLYCERIDES Up to 18 years less than 1.20 mmol/L

Over 18 years less than 1.71 mmol/L

TSH, SENSITIVE (S–TSH) 0.3 – 5.6 mIU/L

UIBC 19 – 51 mol/L

URATE (URIC ACID) Male 180 – 450 mol/L

Female 120 – 400 mol/L

URATE (URIC ACID) URINE Random 1.0 – 3.0 mmol/L

24–hour 1.50 – 4.50 mmol/d

URATE SYNOVIAL FLUID 200 – 480 mol

UREA (BUN) 3.0 – 8.2 mmol/L

VANILMANDELIC ACID

(VMA–URINE) 10.0 – 40.0 mol/d

VALPROIC ACID 347 – 693 mol/L

VITAMIN B12 Deficient < 107 pmol/L

Indeterminate 107 – 133 pmol/L

Sufficient > 133 pmol/L

VITAMIN D (25–HYDROXY) Ideal 75 – 200 nmol/L

Toxic > 250 nmol/L

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HEMATOLOGY DEPARTMENT

TEST GUIDELINES REFERENCE

RANGE UNITS BLOOD FILM (DIFFERENTIAL)

Relative Segmented Neutros 0.40 – 0.75 Frac of 1

Band Neutros 0.00 – 0.05 Frac of 1

Lymphocytes 0.20 – 0.45 Frac of 1

Monocytes 0.02 – 0.13 Frac of 1

Eosinophils 0.01 – 0.06 Frac of 1

Basophils 0.00 – 0.01 Frac of 1

Absolute Neutros 2.0 – 7.5 x10⁹/L

Lymphocytes 1.1 – 3.3 x10⁹/L

Monocytes 0.0 – 0.8 x10⁹/L

Eosinophils 0.0 – 0.5 x10⁹/L

Basophils 0.0 – 0.2 x10⁹/L

D-DIMER < 500 µg/mL

ERCS (RBC) Male 4.5 – 6.5 x10¹²/L

Female 3.8 – 5.8 x10¹²/L

ESR. (SED. RATE) Male:

17 – 50 years 0 – 10 mm

> 50 years 0 – 14 mm

Female:

17 – 50 years 0 – 12 mm

> 50 years 0 – 20 mm

FIBRINOGEN 1.5 – 4.0 g/L

HEMATOCRIT (PCV) Male 0.37 – 0.54 Frac of 1

Female 0.37 – 0.47 Frac of 1

HEMOGLOBIN Male 135 – 180 g/L

Female 115 – 165 g/L

LKCS (WBC) 4.0 – 11.0 x10⁹/L

MCH 27.0 – 32.0 pg

MCHC 320 – 360 g/L

MCV 80 – 97 f/L

RETICULOCYTE COUNT 25 – 85 x10⁹/L

THROMBOCYTES (Platelets) 150 – 400 x10⁹/L

RDW 11 – 14.5

INR 0.8 – 1.2

PTT 26 – 39 Seconds

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MISCELLANEOUS

TEST GUIDELINES REFERENCE

RANGE UNITS

URINALYSIS

(Routine) Bilirubin Negative

Blood Negative

Glucose Negative

Ketones Negative

Leukocytes Esterase Negative

Nitrite Negative

pH 5 – 9

Protein Negative

Specific Gravity 1.005 – 1.030

Urobilinogen 3.2 – 16 mol/L

(Microscopic) WBC 0 – 9 p/uL

RBC 0 – 5 p/uL

Epithelials 0 – 9 p/uL

Epithelials Non-Squamous 0 – 2 p/uL

Casts (hyaline) 0 – 2 p/uL

Mucous 0 – 264 p/uL

Amorphous Phosphates 0 – 6 p/uL

Amorphous Urates 0 – 6 p/uL

Crystals

Calcium Oxalate 0 – 6 p/uL

Urate 0 – 6 p/uL

Triple Phosphate 0 -6 p/uL

Bacteria 0 - 75 p/uL

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PEDIATRIC HEMATOLOGY REFERENCE RANGES

TEST BIRTH 1 WK–2 MO 3–11 MO 1–5 YR 6–10 YR 11–14 YR UNITS

TOTAL LKCS 20 – 40 5 – 21 5 – 15 5 – 12 4 – 11 4 – 11 x10⁹/L NEUTS 6 – 26 1.5 – 10 1.5 – 8.5 1.5 – 8.5 1.5 – 8 2 – 7.5 x10⁹/L

BANDS 0 – 0.8 0 – 0.8 0 0 0 0 x10⁹/L

LYMPHS 2 – 11 2 – 17 4 – 10.5 2 – 8 1.5 – 7 1.1 – 3.3 x10⁹/L MONOS 0.4 – 3.1 0.2 – 2.4 0.05 – 1.1 0 – 0.8 0 – 0.8 0 – 0.8 x10⁹/L EOS 0.02 – 0.85 0.07 – 1 0.05 – 0.7 0.02 – 0.5 0 – 0.5 0 – 0.5 x10⁹/L BASOS 0 – 0.2 0 – 0.2 0 – 0.2 0 – 0.2 0 – 0.2 0 – 0.2 x10⁹/L

ERCS 3.5 – 6 3.5 – 5.5 3.5 – 5 4 – 5 4.5 – 5.5 4.5 – 5.5 x10¹²/L HB 150 – 250 115 – 180 100 – 140 110 – 140 120 – 160 125 – 170 g/L HCT 0.46 – 0.74 0.35 – 0.54 0.30 – 0.42 0.33 – 0.42 0.36 – 0.48 0.39 – 0.50 frac of 1

MCV 95 – 120 77 – 115 70 – 86 80 – 94 80 – 94 80 – 100 fL

MCH 33 – 39 24 – 34 24 – 31 24 – 31 24 – 31 24 – 31 pg

MCHC 320 – 360 320 – 360 320 – 360 320 – 360 320 – 360 320 – 360 g/L RETICS 200 – 300 10 – 100 10 – 100 10 – 100 10 – 100 10 – 100 x109/L PLATELETS 150 – 400 150 – 400 150 – 400 150 – 400 150 – 400 150 – 400 x109/L