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Bacteriology, Parasitology, Mycology & Medical Biotechnology

Time – according to routine

Month Week Day Topics Teacher

August 2

nd

Thurs Introduction & History of Medical Microbiology Prof. Akram Hossain 2

nd

Sat

Bacterial Structure, Nomenclature & classification

Dr. Shyamal Kr Paul 2

nd

Sun Parasitology Terms & definitions classification Dr. Nasir Uddin Mahmud 2

nd

Mon Growth & Nutrition of Bacteria, culture media Dr. Salma Ahmed 2

nd

Tues Structure & physiology of protozoa & Helminths Dr. Santana Sarkar August 4

th

Sat

Principle of isolation and identification of bacteria

Dr. Salma Ahmed

4

th

Sun Trichomonas, Trypanosmoa Dr. Faria ferdous

4

th

Mon Sterilization, Disinfection, asepsis Dr. Arup Islam

4

th

Tues Antimicrobial agents, Dr. Shamsun Nahar

4

th

Wed Drug resistance Prof. Akram Hossain

September

1

st

Sat Host parasite relationship, resident flora and opportunistic bacteria

Dr. Shyamal Kr Paul

1

st

Sun Pathogenesis/ virulence factor of bacteria Dr. Chand Mahmud 1

st

Mon Entamoeba, free living amoeba Dr. Emily Rahman

1

st

Tues Plasmodium Dr. Shyamal Paul

1

st

Wed Staphyloccus Dr. Emily Rahman

1

st

Thurs Opportunistic protozoa, Toxoplasma, B.coli Dr. Mahmudur Rahman

2

nd

Sat Streptococci-1 Dr. Shamsun nahar

2

nd

Sun Streptococci-2 Dr. Shamsun nahar

2

nd

Mon Enterobacteriaceae Prof. Akram Hossain 2

nd

Tues Enterobacteriaceae -2 Prof. Akram Hossain

2

nd

Wed Leishmania Dr. C Mahmud

2

nd

Thurs Mycobacterium tuberculosis Dr. Arup Islam 3

rd

Sat Clostrdium, Anaerobic Bacteria Dr. Shyamal Kr Paul

3

rd

Sun Chlamydia, Mycoplasma Dr. Emily Rahman

3

rd

Mon Intestinal Nematodes-1 Dr. Mahmudur Rahman

3

rd

Tues Intestinal Nematodes-1 Dr. Mahmudur Rahman

3

rd

Wed Tissue Nematodes Dr. Farid Ahmed

3

rd Thurs

Neisseria Dr. N. Mahmud

4

th

Sat Trematodes Dr. Masud

4

th

Sun Rickettssia Dr. Faria Ferdous

4

th

Mon Introduction to Zoonotic bacterial disease Dr. Santana Sarkar

4

th

Tues Cestodes-1 Dr. Ziaur Rahman

4

th

Wed Cestodes-2 Dr. Ziaur Rahman

4

th

Thur Opportunistic mycobacteria Dr. Arup Islam

(2)

Bacteriology, Parasitology, Mycology & Medical Biotechnology

Month Week Day Topics Teacher

October

1

st

Sat Introduction to fungi and antifungal drugs Dr. Santa sarkar

1

st Sun

Dematophytes Dr. Shyamal Kr Paul

1

st

Mon Opportunistic fungi-1 Dr. C.Mahmud

1

st

Tues Opportunistic fungi-2 Dr. C.Mahmud

1

st

Wed Bacillus, C.diphtheriae Dr. Arup Islam

1

st

Thurs Actinomycosis Dr. Farid Ahmed

2

nd

Sat Treponemma Dr.Masud

2

nd

Sun Haemophilus Dr. Ziaur Rahman

2

nd

Mon Listeria, Legionella, Brucella Dr. Farid Ahmed 2

nd

Tues Gardnerella, Morganella, Acinetobacter Dr. Masud

2

nd

Wed Borellia, Leprospira Dr. Ziau Rahman

2

nd

Thurs Helicobacter Prof. Akram Hossain

3

rd

Sat Vibrio, Campylobacter Dr. Santana Sarkar

3

rd

Sun Pseudomonas Dr. Masud

3

rd

Mon Bacillus, Corynebacterium Dr. Farid Ahmed

3

rd

Tues Yersinia Dr. Faria Ferdous

(3)

Bacteriology, Parasitology, Mycology & Medical Biotechnology Academic Calendar for the session 2012

Bacteriology Pactrical Month/We

ek Topic Supervisor Teachers

comment August

2

nd

week

 Orientation in the department and Reception: Patient dealing, sample collection, processing, recording &

reporting system

Prof. Akram Hossain

August 4

th

week

 Aseptic practices in laboratory and safety precautions

 Collection/transport of specimens for microbiological investigations

 Sterilization Section, Lab safety, waste disposal

 Introduction to BSL -1& 2

Dr. Salma Ahmed Dr. Emily Rahman Dr. Nazia Hoque Mr. AHM Rafi

September 1

st

week

 Operation of autoclave, hot air oven, distillation/ de-ionizing plant, filter, Refrigerated centrifuge, Microwave oven, Micropipette, Water bath, Heating block

Dr. Shyamal Kr Paul Dr. N. Mahmud Mr. AHM Rafi

September

2

nd

week  Microscopy (Light, DGI, Phase contrast, Imm FI Microscopy:

Maintenance)

Prof. Akram Hossain Dr.N. Mahmud Dr. Arup Islam Mr. AHM Rafi

 Preparation of stains viz. Gram, Ziehl Neelsen , Albert’s, capsules, spores, (ZN) etc. with SOP

September

3

rd

week  Preparation, examination &

interpretation of direct smears from clinical specimens ( stool, urine, Pus, HVS, Sputum, U/S, CSF)

Dr. C.Mahmud Dr. N. Mahmud Mr. AHM Rafi September

4

th

week  Introduction to Plating of clinical specimens on media for isolation, purification, identification and quantitation purposes.

Dr. C. Mahmud Dr. Sanatana sarkar

September 4

th

week October 1

st

week

 Preparation of media like Nutrient Broth/ agar, Blood Agar, Mac-conkey agar,

 Robertson’s cooked meat broth, Lowenstein Jhensen

 . Preparation of reagents -oxidase, Kovac Sugars, Kligler iron agar, etc.

 Quality control of media, reagents etc.

 Bleeding techniques of sheep and preparation of BA and Chocolate agar

Dr.Shyamal Kr. paul

Dr. Sanatana sarkar

Dr. Faria ferdous

Dr. Shamsun Nahar

Jolly

(4)

Bacteriology, Parasitology, Mycology & Medical Biotechnology Month/We

ek Topic Supervisor Teachers

comment media.

October

2

nd

week  Study of Colony Morphology Of Common Bacteria- Staphyloccus, Strepcococcus, Esch Coli, Salmonella, Shigella, Pseudomonas, Proteus, Klebsiella, Enterococci, Campylobacter, Mycobacterium (Using control strains)

Dr. C. Mahmud Dr. Sanatana Sarkar Dr. Emily Rahman Dr. Faria Ferdous

October

3

rd

week  Tests for Motility, hanging drop, DGI, Soft agar, catalase, oxidase, Coagulase, (slide & tube) Sugar fermentation, TSI, Citrate, Indole, Hippurate etc

Dr. Emily Rahman Dr. Arup Islam Dr. Sanatana Sarkar Mr. AHM Rafi

October

3

rd

week  Special culture technique including anaerobiosis, Microaerophilic etc

Dr. Emily Rahman Dr. Arup Islam Dr. Sanatana Sarkar Mr. AHM Rafi October

4

th

week  Identification Of Bacteria from clinical specimen Of Medica1 Importance Upto Species Level including serotyping.

Dr.Shyamal Kr. paul Dr. Sanatana sarkar Dr. Faria ferdous Mr. AHM Rafi November

1

st

week  Quantitative analysis of urine by pour plate method and semi quantitative analysis by standard loop tests for finding significant bacteriuria

Dr.Shyamal Kr. paul Dr. Sanatana sarkar Dr. Faria ferdous Mr. AHM Rafi November

2

nd

week  Culture of Blood, Sputum, CSF, Stool etc.

Dr. Salma Ahmed Dr. Sanatana sarkar Dr. Faria ferdous Mr. AHM Rafi November

2

nd

week  Maintenance & preservation of bacterial cultures

Dr. Salma Ahmed Dr. Sanatana sarkar Dr. Faria ferdous Mr. AHM Rafi November

3

rd

week  Efficacy of sterility by autoclaving , Hot air oven and filtration

Prof. Akram Hossain Dr. Emily Rahman Dr. Arup islam November

3

rd

week  Bacteriological study of Mobiles, key boards, table top.

Prof. Akram Hossain

Dr. Emily Rahman

Dr. Arup islam

November  Introduction to Molecular techniques- Dr.Shyamal Kr. paul

(5)

Bacteriology, Parasitology, Mycology & Medical Biotechnology Month/We

ek Topic Supervisor Teachers

comment 4

th

week

December 1

st

 DNA/ RNA extraction from clinical specimen, Bacterial colony

 Different type of Electrophoresis for detection DNA/RNA

 Amplification of DNA

 Documentation of amplicon.

Dr. Sanatana sarkar Dr. Faria ferdous Mr. AHM Rafi

December

2

nd

week  Visit of BSMMU, BIRDEM, IPH Prof. Abu Saleh Prof. JA Ashraful Haque

Parasitology

Month/week Section Supervisor Teachers

comment August

4

th

week

 Examination of faeces for parasite ova and cysts etc. by direct Microscopy and concentration methods (salt floatation and formol-ether methods)

Dr. Emily Rahman Dr. Mahmudur Rahman Mr. AHM Rafi

August

4

th

week  Egg counting techniques for helminths by Macmaster technique

Dr. Emily Rahman Dr. Mahmudur Rahman Mr. AHM Rafi

 Preservation of parasites- mounting, fixing, staining etc.

Dr. Emily Rahman Dr. Mahmudur Rahman Mr. AHM Rafi

September

1

st

week  Examination of blood for protozoa and helminths by wet mount, thick

 and thin stained smears

 Examination of blood for microfilariae including concentration techniques

Dr. C Mahmud

Dr. Mahmudur Rahman Dr. Masud

September

2

nd

week  Culture media Leishmania donovani in NNN medium

 Culture of Helminths by Haradimori technique

 Preparation of stains- Giemsa, Leishman, Methylene blue, Fields stain, Iron hematoxylene,

Dr. C Mahmud

Dr. Mahmudur Rahman

Dr. Masud

(6)

Bacteriology, Parasitology, Mycology & Medical Biotechnology etc

Mycology

Month/week Section Supervisor Teachers

comment September

3

rd

week

 Collection and transport of specimens for diagnosis of Fungal diseases

 Collection technique for dermatophytes from Skin, nail and Hair

Dr. Shyamal kr paul Dr. Ziaur Rahman Dr. farid Ahmed

September 3

rd

week

 Processing of samples for microscopy and culture

 o Direct examination of specimens by KOH, Gram’s, Acid fast,

 Giemsa, Lactophenol cotton blue

& special fungal stains

Dr. Shyamal kr paul Dr. Ziaur Rahman Dr. farid Ahmed

September

4

th

week  Isolation and identification of medically important fungi &

common laboratory

contaminants

Dr. N.Mahmud Dr. Emily Rahman Dr. Shamsun Nahar

 Comparison of SDA and Special fungal media like Dermatophyte test media alike

 Germ tube test for Candida

Dr. N.Mahmud Dr. Emily Rahman Dr. Shamsun Nahar October

1

stth

week  Special fungal culture techniques e.g. Plates culture, Slide culture

Dr. Shyamal Kr Paul Dr. Sanata Sarkar

 Special techniques like hair baiting, hair perforation, paraffin baiting and

(7)

Bacteriology, Parasitology, Mycology & Medical Biotechnology

Virology

Duration

in Week Topic Supervisor Teachers comment

one  Preparation of clinical specimens for isolation of viruses

 Collection & transport of specimens Two  Serological tests -ELISA for

HBsAg, HBsAB, HBeAg, HCV, HEV

 ICT for HIV, HCV , HEV etc One  Molecular techniques in virology

 Polyacrylamide Gel electrophoresis for Rota virus

 Introduction to cell/ Tissue culture

 Preparation of glassware for tissue cultures (washing, sterilisation).

 Preparation of buffers like PBS, Hank’s, TBE

Water Bacteriology

Duration in Week

Topic Supervisor Teachers comment

one  Examination of Water –

o Sampling – Tap water, River water, Tube well water

o Counting indicator bacteria- Fecal Esch coli

o Filtration method o Multiple tube - MPN Two  Examination of Milk –

o Methylene blue test

o Phosphatase test

One  Food Bacterilogy

(8)

Bacteriology, Parasitology, Mycology & Medical Biotechnology

Book List for the session 2012

Bacteriology

1. Medical Microbiology,LANGE

2. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases

3. Medical Microbiology Patrick. M. Murry

4. District Laboratory Practice in Tropical Countries; Part 1 and Part II;

Monica Cd Cheesbrough, Cambridge university press. Reprint 2007 5. Mackie & McCartney ; Practical Medical Microbiology, Fourteenth

Edition, Churcill Livingstone

Immunology

1. Medical Immunology, LANGE

2. Immunology ; Richad A. Goldsby, Janis Kuby 3. Roitt’s Essential Immunology

Parasitology

1. K. D. Chatterjee

2. Medical Parasitology basic and clinical.

Mycology

1. Medically Important Fungi, 4th Edition, Davise H Larone, ASM press, Washington DC

2. Human Mycology by Dr. Ahmed Abu Saleh

Distribution: Dr. Chand Mahmud, Dr. S.K Paul Dr. Salma Ahmed, Dr. Nazia Haque, Dr. N Uddin Mahmud, Dr. Arup Islam, Dr. Emily Rahman, Dr. Santana sarkar, Dr.

faria Ferdous, Dr. Mahmudur Rahman, Dr. Shamsun Nahar, Dr. Farid Ahmed, Dr.

Ziaur Rahman, Dr. Masud ur Rahman.

References

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