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Hsp72 is targeted to the mitotic spindle by Nek6 to promote K fiber assembly and mitotic progression

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Academic year: 2020

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Figure

Figure 1. Nek6 interacts with and phosphorylates Hsp72.were prepared from HEK 293 cells transfected with Flag-Nek6 and synchronized in S or M
Figure 2. Hsp72 promotes chromosomes congression and mitotic progression. (A) HeLa:EGFP–lamin A/mCherry-H2B cells were either mock-depleted, depleted with siRNAs against Hsp72 for 72 h, or treated with Hsp70i for 4 h, as indicated, before time-lapse imagin
Figure 3. Hsp72 promotes recruitment of ch-TOG–TACC3 complexes to K-fibers. (A) HeLa cells treated as indicated were placed on ice for 10 min before IF with -tubulin and CENP-A antibodies to reveal K-fibers
Figure 4. Nek6 phosphorylation targets Hsp72 to the mitotic spindle.(SBD) binding domains and sequence alignment of the human Hsp70 family around threonine-66
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