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Original Article An optimized protocol for whole mount in situ hybridization of mouse brain

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Academic year: 2020

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Figure

Figure 1. Fluorescence in situ hybridization for c-fos mRNA in hippocampus. (A-D) show the c-fos mRNA expression of 20 μm, 40 μm, 80 μm, and 100 μm, respectively
Figure 2. Representative fluorescence in situ hybridization results. (A) Repre-sentative confocal image from 20 μm sections showing c-fos mRNA labeling in auditory cortex, Scale bar = 50 μm
Figure 3. The influence of different times digested with proteinase K to the results of double labeling for c-fos protein (green) and mRNA (red) in mouse auditory cortex

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