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Development of Colorimetric Microtiter Plate Assay for Assessment of Antimicrobials against Acanthamoeba

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Figure

FIG. 1. Reduction of alamarBlue by different plating densities of A. castellaniialamarBlue were achieved by 5.0triplicate culturesculture volume, up to 5200� 10 (a) and A
FIG. 2. Reduction of alamarBlue by different plating densities of A.10triplicate culturescastellanicastellanii (b) cultured in 10096 h (open squares), 72 h (closed squares), 48 h (open circles), and 24 h(closed circles) prior to the addition of alamarBlue
FIG. 4. Relative susceptibilities of A. castellaniiand (closed squares) A. polyphaga (open circles) to the following over 96 h: (a) octa-

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