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Vascular endothelial growth factor coordinates islet innervation via vascular scaffolding

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Figure

Fig. 1. Islet innervation follows islet VEGF productionand vascularization. TUJ1group
Fig. 2. Islet VEGF production influences bothsympathetic and parasympathetic innervation.Scale bars: 100 PECAM1 (green) and either TH (marker of sympatheticinnervation; red in A-C, grayscale in Aacetylcholine transporter (VAChT; marker ofparasympathetic innervation; red in D-F, grayscale in D(A-F′) Representative islets from adult (>10-week-old)control (A,D), Pdx1-Cre;Vegfafl/fl (VEGFDown; B,E), anddoxycycline-treated (for one week) RIP-rtTA;TetO-hVegfa(VEGFUp; C,F) mice, immunolabeled for insulin (blue),′-C′) or the vesicular′-F′).μm.
Fig. 3. Developing islet clusters areinterconnected by the neuronal network. (A-C) Images are 3D reconstructions of arepresentative confocal z-stack (100 μm thick) ofE14.5 pancreas from a control mouse, immunolabeledin whole mount
Fig. 4. VEGF is not required for, but enhances, pancreaticinnervation during embryogenesis.mice
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