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Attenuation of Peripheral Regulatory T Cell Suppression of Skin Homing CD8+T Cells in Atopic Dermatitis

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Figure

Fig. 1. The purities of isolated Tregs (CD4+CD25+T cells). PBMCs were iso-lated on Ficoll-Hypaque gradient
Fig. 2. Frequencies of peripheral CD8+CLA+T cells. The frequencies of CD8+CLA+T cells in peripheral blood were measured by flow cytometry, and the repre-sentative results (A) from AD patients and healthy volunteers were shown by dot plot graphs
Fig. 4. Proliferation of peripheral CD8+CLA+T cells. CFSE-labeled peripheral CD8+CLA+T cells stimulated with anti-CD3/CD28 in the absence of Tregs for 4 days
Fig. 7. Suppression of CD8+CLA+T cells proliferation by Tregs is mediated by TGF-β1. 5×104 Tregs co-cultured with 5×104 autologous CD8+CLA+T cells from AD patients (n=8, gray bars) or healthy volunteers (n=8, white bars) stimulated with anti-CD3/CD28 in 96

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