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Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis by High Resolution Melting Analysis

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Figure

TABLE 1. Primer and probe sequences used for PCR
TABLE 2. Primer sequences used for sequencing of a blinded series of 59 M. tuberculosis clinical isolates
TABLE 3. Reference strains with known mutations used forassay development
FIG. 1. High-resolution melt curves of katGand (A), the mabA promoter (B), inhA (C), the ahpC promoter (D), the ahpC promoter (probe) (E), rpoB (F), demonstrating the change in melt curve shape caused by mutations
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