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Perfectly matched 20 nucleotide guide RNA sequences enable robust genome editing using high fidelity SpCas9 nucleases

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Figure

Fig. 1 Analysis of the on-target activities of wild-type (WT) SpCas9 and three high-fidelity SpCas9 variants for seven genomic sites using differentsgRNAs
Fig. 2 Comparisons of the off-target editing activities of WT SpCas9 and three high-fidelity SpCas9 variants at five off-target sites with sgRNAsproduced from U3:tRNA–sgRNA constructs and U3:sgRNA constructs
Fig. 3 Comparisons of the specificities of WT SpCas9 and eSpCas9(1.0), eSpCas9(1.1), and SpCas9-HF1 with tRNAeffects of WT SpCas9 and variants eSpCas9(1.0), eSpCas9(1.1), and SpCas9-HF1 with guide sequences containing pairs of mismatches at successiveposit

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