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Optimizing complex phenotypes through model guided multiplex genome engineering

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Figure

Fig. 1 Workflow for improving phenotypes through model-guided multiplex genome editing
Fig. 2 Mutation dynamics over many cycles of MAGE allele reversion. a Increase in combinatorial diversity and reversion count vs
Fig. 3 Genotypic and phenotypic diversity in 87 clones sampled across 50 MAGE cycles enabled model-guided prioritization of top single nucleotide variants(SNVs) for further validation
Fig. 4 Construction and characterization of final strain C321.suspended in phosphate buffered saline (PBS) for each sample and normalizing to the fluorescence ratio of non-recoded strain EcNR1.mutS.KO expressing 0ΔA.opt
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