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In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila

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Figure

Figure 1 dCas9-VPR activates target gene expression in Drosophila S2R+ cells. (A) Schematics of the constructs tested in this study
Figure 2 Effects of individual sgRNA on target gene activation. (A) Three nonoverlapping sgRNAs tiling the region upstream of a QUAS:luciferasetested singly and in combination
Figure 3 Identiwith previous ChIP data. The(A),term enrichment forproportion of differentially expressed genes that also show ChIP peaks forfication of snail and twist target genes using dCas9-VPR and RNAseq
Figure 4 In vivopouch (white arrow). Seemargin (open arrowhead). (B and Bexpression of UAS-3X-FLAG:dCas9 activators
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