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Improved proliferation of antigen-specific cytolytic T lymphocytes using a multimodal nanovaccine

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Figure 1 Design and synthesis of a novel αgalcer analog PBs-44.Notes: (a) Ph3P=CH(CH2)12ch3, THF (60%); (b) OsO4, N-methylmorpholine N-oxide, t-BuOh, h2O (70% for 3, 28% for 4); (c) HCl, THF; MeOH; HO2C(CH2)12ch=CH(CH2)7chdicyclohexylcarbodiimide, N-hydroxysuccinimide, THF, dimethylamino pyridine (47%); (d) sodium methoxide, MeOH; tert-butyldimethylsilyl chloride, imidazole, THF (57%); 3,(e) AC2O, TFA; HBr, AcOH, methylene chloride (40%); (f) AgOTf, 4Å mol sieves, methylene chloride, H2, Pd/C, EtOAc, EtOH, sodium methoxide, MeOH (85%).Abbreviation: αgalcer, α-galactosylceramide.
Figure 2 Physical properties of the PLGA-based NanoVac.Notes: (A) Optical image of the NanoVac
Figure 3 The controlled release of NanoVac and characterization of PBS-44 in DCs.Notes: (A) Controlled release of ova antigen from NanoVac in physiological pH over a 25-day period
Figure 5 (Continued)
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