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Anti-HER2 antibody and ScFvEGFR-conjugated antifouling magnetic iron oxide nanoparticles for targeting and magnetic resonance imaging of breast cancer

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Figure 1 antibody conjugation of nanoparticles with polysiloxane copolymer coating. Abbreviations: PeO, poly(ethylene oxide); IONPs, iron oxide nanoparticles; aPTes, (3-aminopropyl) triethoxysilane; sulfo-lc-sPDP, sulfosuccinimidyl 6-[3′(2-pyridyldithio)-propionamido] hexanoate; DTT, dithiothreitol.
Figure 2 Prussian blue staining of her2-positive sK-Br-3 cancer cells (A), 100 times free pre-her2 blocked sK-Br-3 cancer cells (B), negative McF-7 and MDa-MB-231 cancer cells (C and D) treated with anti-her2-IONPs, and both sK-Br-3 and MDa-MB-231 treated
Figure 3 Prussian blue staining of MDa-MB-231 cancer cells bound with scFvegFr-IONPs (A), 100 times free scFvegFr inhibited the binding of egFr-positive MDa-MB-231 cancer cells (B), negative McF-7 and MDa-MB-453 cancer cells (C and D) treated with scFvegFr
Figure 4 Transmission electronic microscopic images of uptake of scFvegFr-IONPs by MDa-MB-231 cancer cells (A)
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