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Heparan sulfate is an important mediator of Ebola virus infection in polarized epithelial cells

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Figure

Fig. 1 Establishment of a polarized Caco-2 cell monolayer. a Caco-2 monolayers were seeded at a density of 4 × 104 and allowed to grow for10 days after seeding
Fig. 2 Basolateral infection of EBOV is more efficient in Caco-2 cells a Caco-2 cells infected with EBOV at 3 pfu/cell were assessed for EBOV RNAexpression at 6, 24, and 48 hpi, using SYBR-green qPCR assay and normalized to GAPDH expression
Fig. 3 Extent of cell polarity affects cell susceptibility to EBOV infection. Caco-2 cells were infected on 4, 6 or 8 days post-seeding at 3 pfu/cell.Cells were assessed for EBOV RNA expression at 6, hpi, using SYBR-green qPCR assay and normalized to GAPDH
Fig. 4 Semiconfluent monolayers are more susceptible to apicalEBOV infection. Caco-2 cells were grown to either semiconfluence(day 4 pi) or confluence (day 6 pi) and infected with EBOV at 3 pfu/cell
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