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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy

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Figure

Figure 1. (A) Sample measured decay trace (blue dotted line) and the fitting curve (red line) from a pixel in data at 2.5 hours of incubaton
Figure. 3. Sample time-lapse average lifetime images of Photofrin® (20 µg/mL) uptake by MLL cells between 1-18 hours
Figure 4. Distribution of τ2 (slow component) as a function of different incubation times
Figure 6. Distribution of A2 as a function of different incubation time. A2 was increased from 19% to 35% as cells took up more Photofrin®as the incubation time increased, suggesting contribution of τ2 (mostly from photoproducts of Photofrin® under strong
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