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Osteoarthritic cartilage explants affect extracellular matrix production and composition in cocultured bone marrow-derived mesenchymal stem cells and articular chondrocytes

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Figure 1 Cell culture models. (A) Bone marrow-derived stem cells (BMSCs), mixed cultures (BMSCs and chondrocytes in a ratio of 1:1), andchondrocytes were embedded in fibrin gels and applied onto the surface of osteoarthritis articular cartilage explants (c
Figure 2 Quantification of gene expression with quantitative polymerase chain reaction.COL2A1(BMSCs) (white bars), mixed cultures (BMSCs and chondrocytes in a ratio of 1:1, light grey bars), or chondrocytes (dark grey bars) were kept asmonocultures (F, bar
Figure 3 Quantification of collagens I, II, and III and glycosaminoglycan (GAG) in fibrin gel lysates.chondrocytes in a ratio of 1:1, light grey bars), or chondrocytes (dark grey bars) were kept as monocultures (F, bars with pattern) or as co- andtri-cultu
Figure 4 Immunofluorescence staining of collagens. Representative immunofluorescence staining images of collagens (A) I, (B) II, (C) III, and(D) X detected on cryosections after 28 days of culture
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