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A method for measuring electrical signals in a primary cilium

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Figure

Figure 1 Culture system for the growth of ciliated cells on beads.di-8-ANEPPS (di-8). (the ciliary marker acetylated (A-B) Phase-contrast image of live cells on a bead with contrast set toshow cell bodies (A) or cilia (B)
Figure 2 Photomicrograph of the method for electricalrecording from an isolated primary cilium
Figure 3 Voltage-clamp recordings from single renal primary cilia. All recordings were made in the standard recording solutions except asnoted

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