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Figure

Fig. 1. Structure of the cloning mRNA ment. (1) 5’-untranslated region (5’-UTR) contains the IRES sequence, (2) leader protein sequence, (3) exon sequences, (4) 3’-untranslated region (3’-UTR), ATG  – start codon
Fig. 2. Electrophoretic separation of digestion plas-mids extracted from five transformant clones
Fig. 4. Limb salvage in patent who underwent intra-muscular gene transfer of naked plasmid encoding VEGF165: (a) before treatment, (b) 6 weeks after gene transfer, (c) 12 weeks after gene transfer
Fig. 5. Limb salvage in patent who underwent combine cell/gene transfer of naked plasmid
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