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miR 200–containing extracellular vesicles promote breast cancer cell metastasis

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Academic year: 2020

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Figure

Figure 1. miR-200 microRNAs are secreted in EVs from 4T1E cells. (A) Size distribution of EVs released by 4TO7 and 4T1E cells in 48-hour culture super-natants (3 experiments)
Figure 2. miR-200 miRNAs are transferred from metastatic 4T1E cells to poorly metastatic 4TO7 cells
Figure 3. miR-200 miRNAs are transferred across a Transwell or by EVs. (A) miRNA and mRNA levels in 4TO7 cells, untreated or cocultured for 6 days with 4TO7 or 4T1E cells at a 1:4 ratio separated by Transwell
Figure 4. Transferred miR-200c associates with donor CD63 and AGO2. (A) Schema of 4T1E cells transfected with CD63-RFP plasmid and Cy5–miR-200c and cocultured with 4TO7-GFP cells
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