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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus

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Figure

TABLE 1. Sequences of FRET probes and primers used in thedengue virus real-time RT-PCR assays
FIG. 1. Alignment of the 3�serotype is represented by a Singaporean and two foreign isolates
TABLE 2. Sensitivity of each RT-PCR assay: SYBR green pan-dengue RT-PCR, serotype-specific duplex assay, and serotype-specific fourplex assay based on PFU per reactiona
TABLE 5. SYBR green RT-PCR assay

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