The world's largest elearning website for analytical scientists

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e-Learning for Analytical Chemists

www.chromacademy.com

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CHROMacademy will help increase your knowledge, efficiency and productivity in the lab

The world's largest eLearning website for analytical scientists

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GC

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A CHROMacademy Premier Membership provides you with complete access to all content including:

6 New Video Training Courses

Fundamentals of HPLC / GCMS / GC / LCMS Method Development - HPLC & GC

1000’s of eLearning Topics

HPLC / GC / Sample Prep / MS/ Infrared / Basic Lab Skills* / Biochromatography* /

* coming in 2015

HPLC & GC

Troubleshooting and

Virtual Lab Tools

Become the lab expert with our HPLC and GC troubleshooters

Application Notes & Articles

The latest application notes & LCGC articles

24hr Chromatography Support

Ask the Expert - our experts will answer your questions within 24 hours

Assessments

Test your knowledge, certificates awarded upon completion

The Essential Guide

Webcast & Tutorial Archive

Over 70 training topics covered by industry experts

User Forum

Communicate with others interested in analytical science

Lite members have access to less than 5% of our content... Premier members get so much more

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e-Learning for analytical scientists

Used by over 35,000 chromatographers worldwide, CHROMacademy will help increase your knowledge, efficiency and productivity in the lab. With a vast library of high-quality animated and interactive e-learning topics, webcasts, tutorials and troubleshooting tools we can help you refresh your chromatography skills or learn something completely new.

CHROMacademy is simply the best training tool for analytical scientists - see for yourself!

Packed with practical information that will help

improve your skills and productivity

e-Learning for analytical scientists

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MS

GC

HPLC

IR

BLS BIO

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Each channel is packed with e-learning modules,

webcasts, tutorials and interactive tools

HPLC | GC | MS | Sample Prep | Infrared

Basic Lab Skills* | Biochromatography*

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High Performance Liquid

Chromatography

THE THEORY OF HPLC • Introduction to HPLC • Chromatographic Parameters • Band Broadening • Column chemistry

• Reverse phase (partition) chromatography • Ion-Pair Chromatography

• Normal phase (absorption) chromatography • Gradient HPLC

• Quantitative and Qualitative HPLC • Fast HPLC

• HILIC

• Supercritical Fluid Chromatography • Introduction to Ion Chromatography

INSTRUMENTATION OF HPLC • Mobile Phase Considerations • HPLC Solvent Pumping Systems • Autosamplers

• Detectors

e-Learning for Analytical Chemists

Stator

Rotor Seal Isolation Seal

Stator Face

It is necessary to divert the flow of mobile phase away from the sampling system (i.e. the syringe) when aspirating the sample and / or filling the sample loop prior to injection. This is achieved using the injection valve containing a Rotor Seal. The interface between the HPLC capillaries and the stator is known as the Stator Face...

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Gas Chromatography

THEORY AND INSTRUMENTATION OF GC • Introduction to GC

• Chromatographic Parameters • Band Broadening

• Gas Supply and Pressure Control • Sampling Techniques

• Sample Introduction • GC Columns

• GC Temperature Programming • GC Detectors

• Supercritical Fluid Chromatography 1. Aims & Objectives

2. Introduction

3. Supercritical Fluids

4. Modes of Chromatography 5. SFC Applications

6. SFC Instrumentation 7. Packed & Capillary SFC 8. The Mobile Phase 9. Exhaust Gases 10. Organic Modifiers 11. Pumping Issues 12. SFC Columns

13. SFC Stationary Phases & Column Selection 14. Detection

15. Pressure Regulators

16. Advantages and Disadvantages of SFC

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Mass Spectrometry

FUNDAMENTAL LC-MS • Introduction

• Electro Spray Ionization Theory

• Electro Spray Ionization –Instrumentation • Mass Analyzers

• Atmospheric Pressure Chemical Ionization (APCI)

• Atmospheric Pressure Photo Ionization (APPI) • Solvents, Buffers and Additives

• Vacuum Systems

• Flow Rates and Flow Splitting • Orbitrap Mass Analyzers FUNDAMENTAL GC-MS • Introduction

• GC Considerations • GC -MS Interfaces MS INTERPRETATION

• General Interpretation Strategies

• Fundamentals of MS Proteomics Research

e-Learning for Analytical Chemists

Corona Electrode Pin

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Non-Volatile Analyte is Charged in Solution

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Nebulising Gas

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Sample Preparation

SOLID PHASE EXTRACTION • Molecular Properties

1. Learning Aims & Objectives 2. Functional groups

3. Molecular Properties

4. Functional group interactions 5. Hydrophobic or Non-Polar Groups 6. Hydrophobic Interactions -Solubility 7. Hydrophobic Interactions -Sorbents 8. Polar Groups

9. Polar Interactions -Solubility 10. Polar Interactions -Sorbents 11. Ionic groups

12. Ionic groups -pH | Ka | pKa | Ionic strength 13. Ionic Interactions -Solubility | Sorbents 14. Ionic Interactions -Chelating Groups 15. Chelating Groups 16. Chelating Interactions 17. Solubility 18. Sorbents 19. Protein Binding 20. Assessment • SPE Overview • SPE Mechanisms • Method Development

• Primary Sample Preparation Techniques • Liquid / Liquid Extraction Techniques • Approaches to Automation for SPE

e-Learning for Analytical Chemists

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Infrared Spectroscopy

INFRARED SPECTROSCOPY • Introduction to IR Spectroscopy 1. Electromagnetic Spectrum 2. Electromagnetic Radiation 3. Infrared Regions 4. Molecular Vibrations

5. Calculation of Molecular Vibrations 6. Infrared Active Modes

7. Absorption Considerations 8. The IR Spectrum

9. Infrared Absorption Signals 10. Dispersive IR Instruments 11. FTIR Instruments

12. FTIR Operation 13. Sample Preparation

14. Attenuated Total Reflectance (ATR) 16. Applications of IR Spectroscopy 17. References

18. Assessment

• Infrared Spectral Interpretation 1. Infrared Spectral Quality 2. Orbital Hybridization 3. Interpretation Concepts 4. IR Frequencies 5. Alkanes 6. Alkenes 7. Alkynes 8. Aromatic Compounds...

e-Learning for Analytical Chemists

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e-Learning for Analytical Chemists

Basic Lab Skills

coming spring 2015

BASIC LAB SKILLS . Laboratory Safety . Errors

. Volumetric Lab Equipment . Balances

. Measuring pH . Titrations

. UV-visible Spectroscopy

1. Bonding Theory for UV-visible Spectroscopy

2. The Electromagnetic Spectrum 3. Origin of UV-Visible Spectra 4. Transmittance and Absorbance 5. Selection Rules and Intensity 6. UV-visible Chromophores 7. UV-visible Absorption Maxima of Chromophores

8. UV-visible Definitions 9. UV-visible Instrumentation 10. Optical Arrangements of Spectrophotometers

11. UV-Visible Instrument Configuration 12. Key UV-Visible Instrument Parameters

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The location of a balance is important to ensure accuracy when weighing. Manufacturers will have specifications regarding the correct location of a balance within the laboratory.

Draughts

Balances should be located in a draught free location. This includes draughts from doors, windows, air conditioning, passers-by, and other equipment. Draughts will result in fluctuations in the balance reading. Many analytical balances will have doors to shield the weighing plate from draughts...

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Bio Pharmaceutical Analysis

coming summer 2015

BIO PHARMACEUTICAL ANALYSIS . Introduction

1. Global Pharmaceutical Market Overview

2. Basics of Protein BioChemistry – Amino Acids 3. Basics of Protein BioChemistry – Proteins 4. Monoclonal Antibiodies (mAb) in

Bipharmaceuticals

5. Monoclonal Antibiodies – Form and Function 6. Protein Development and Manufacture

7. Biopharmaceuticals – Advances in Efficacy and Safety

8. Trastuzumab – a model monoclonal antibody (mAb)

9. Trastuzumab – production and characteristics 10. Trastuzumab – common post translational modifications

11. Common mAb Post Translation Modifications (PTM’s)

12. Analytical Characterisation at the Protein Level 13. Reversed Phase HPLC Analysis of

Intact and Reduced Proteins 14. Chromatographic Requirements 15. Chemical Derivatisation

e-Learning for Analytical Chemists

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Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc.

Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge...

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Watch, listen and learn with

our new video courses.

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Video Training Courses

Fundamentals of HPLC (Dr. Dawn Watson)

Fundamentals of GCMS (Tony Taylor)

Fundamentals of GC

(Tony Taylor & Amy Claydon) Fundamentals of LCMS (Tony Taylor & Amy Claydon) HPLC Method Development (Tony Taylor)

GC Method Development (Tony Taylor)

Each course contains 4 video training sessions, released over 4 weeks, with full tutor support and certification.

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Member Overview

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QA/QC

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Teaching

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Education

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BA/BS/BSC: 56%

Years of Experience

10+: 45%

6-10: 25%

1-5: 30%

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CHROMacademy contains a vast library of high-quality

animated and interactive e-learning topics

Interactive Learning

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Search for things...

1000’s of eLearning topics, articles and applications

HPLC Troubleshooter

GC Troubleshooter

Fix things...

interactive tools for chromatographers

We developed our Troubleshooters with busy chroma-tographers in mind.

In 3 simple steps you overcome your instrument, separation and quantitation issues.

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Select up to 3 Chromatographic

Symptoms

Positive baseline drift Negative baseline drift Irregular baseline drift

Cycling Baseline (Short Term) Cycling Baseline (Long Term) Erratic baseline

Regular baseline noise Irregular baseline noise Baseline Spikes

Not the Chromatogram I was expecting! Loss of resolution (some peaks)

Loss of resolution (all peaks) No Peaks

Additional peaks (ghost peaks)

Negative peaks (One or more peaks) Peak broadening (Loss of Efficiency) Peak fronting (one or more peaks) Peak shouldering (one or more peaks) Peak splitting (one or more peaks) Peak tailing (one or more peaks) Rounded peaks

Change in selectivity Decreasing retention times Increasing retention times Too much retention

Select up to 2 Instrument

Symptoms

Low pressure High pressure

Continuously decreasing pressure (no sample injection)

Continuously decreasing pressure (after sample injection)

Continuously increasing pressure (no sample injection)

Continuously increasing pressure (after sample injection)

Pressure fluctuation

Leaks in the solvent reserviour connective tubing

Leaks at the degasser / fittings Leaks around the pump head

Leaks around the check valves or fittings Leaks in the autosampler or

connective tubing

Leaks around the sampling valve Leaks at the column fittings Leaks from the detector fiitings Leaks within the detector Poor quantititive reproducibility Poor linearity of response

Possible Causes

Solution

Resources

Mobile phase pH affects retention of ionisable analytes and the selectivity of separations in reversed phase HPLC

Some methods require a very precise pH in order to remain robust

Low pH tends to increase retention of acidic an-alytes and decrease retention of basic anan-alytes

1. Incorrect / non-optimal mobile phase pH 2. Incorrect column geometry / dimensions 3. Buffer precipitation

4. Incorrect buffer / non-optimal buffer concentration 5. Analyte-stationary phase secondary interaction (e.g.

silanol interactions)

6. Incorrect mobile phase

7. Incorrect mobile phase temperature

Buffers and pH Meter

This video explains how to prepare a buffer and use a pH meter.

Buffer preparation

This useful tool will help you to prepare the correct buffer for your HPLC analysis

How to calibrate pH meter

This video describes how to calibrate your pH meter

Normal

Problem

HPLC Troubleshooter

GC Troubleshooter

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Ask The Expert

Can’t find the answer to your question? Ask our panel of experts with a combined expe-rience of over 100 years in chromatography. 24 hours is all we need to answer your ques-tion. Most often, we’ll get something to you before then.

Complex scenarios welcome!

Problem solved!

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Socialize

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Webcasts

Application Notes

Tutorials

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Each topic is covered with a 1hr webcast and

comprehensive tutorial.

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CHROMacademy newsletter

Tips, tricks and practical no nonsense solutions from

chromatography experts each month

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Certified courses with tutor

support.

HPLC Operator Course

HPLC Method Developer Course GC Operator Course

GC Method Developer Course

Each course covers all the key topics and is created by our experts in analytical chemistry. You can take a course at your own pace without a tutor if you wish, or you can opt in to have full tutor support and agree a timescale to complete the course on.

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Reporting Tools.

With a multi user account you can set learning

goals & track user progress

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Saving Time & Money

Scientist “A”

needed to understand why her chromatogram contained baseline spikes combined

with a loss of sensitivity. She turns to CHROMacademy’s HPLC Troubleshooter and in 2.3 seconds,

CHROMacademy tells her there are 26 possible causes for this combination of symptoms.

Each possible cause is ranked using our unique ‘expert system’ and includes a comprehensive

explanation and suggested fixes backed by 100’s of resources from LCGC magazine,

CHROMacademy and instrument vendors.

Without a CHROMacademy Premier Membership:

Called her manufactures’ representative.

36 hours before someone called back

Lost time of instrument use: 40 hours

Lost $ = $2,000 (@$95K/annum)

Contacted her Lab Manager

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Saving Time & Money

Scientist “B”

needed to develop a sensitive GC-MS method for a chlorinated pesticide derivative

present in potable water samples at very low levels. The derivative was new and therefore no

applications existed in manufacturers literature.

Using a CHROMacademy Webcast and Tutorial he was able to develop a GC method from a

generic screening method and realized that Chemical Ionization was the route to attain the required

sensitivity. From the Sample Preparation Channel he noted that solid phase extraction could be

used to selectively extract the target analyte and again was able to develop a method from a

suggested generic starting procedure. He used the ‘ask the expert’ function to enquire of the

CHROMacademy academic team how to create a sensitive MS acquisition method whilst checking

for the presence of co-eluting interferences. Method was developed and was ready for validation

within 1 week.

Without a CHROMacademy Premier Membership:

He wished that the department GC-MS expert hadn’t been laid off…..

Method development now begins using ‘guesstimates’

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