KLARISSA D. HARDY, Ph.D.
Assistant ProfessorDepartment of Pharmaceutical Sciences Lipscomb University College of Pharmacy
One University Park Drive Nashville, TN 37204
615-966-7011 [email protected]
EDUCATION:
Vanderbilt University, Nashville, TN Ph.D., Pharmacology – May 2011
School of Medicine
Department of Pharmacology
Mentors: Jason D. Morrow, M.D. Professor and Chief, Division of Clinical Pharmacology L. Jackson Roberts, II, M.D, Professor of Pharmacology and Medicine
Dissertation title: Formation and Metabolism of 15-Deoxy-Δ12,14-Prostaglandin J2in Vivo
Jackson State University, Jackson, MS B.S., Chemistry – May 2006
College of Science, Engineering, and Technology ACS Certified
Department of Chemistry
PROFESSIONAL APPOINTMENTS:
Lipscomb University, Nashville, TN June 2013 – present
Assistant Professor of Pharmaceutical Sciences College of Pharmacy and Health Sciences
University of Washington, Seattle, WA August 2011 – May 2013
Postdoctoral Research Associate in Department of Medicinal Chemistry Mentors: Allan E. Rettie, Ph.D., Professor and Department Chair *Sidney D. Nelson, Ph.D., Professor and Dean Emeritus
(*Research training began with Dr. Sidney Nelson before his passing Dec. 2011)
Vanderbilt University, Nashville, TN May 2011 – July 2011
Research Associate in Division of Clinical Pharmacology
Supervisor: Ginger L. Milne, Ph.D., Eicosanoid Core Lab Director
Vanderbilt University, Nashville, TN August 2006 – May 2011
Graduate Research Assistant in Department of Pharmacology Mentors: L. Jackson Roberts, II, M.D, Professor
*Jason D. Morrow, M.D. Professor and Division Chief
(*Research training began with Dr. Jason Morrow before his passing July 2008)
Merck Research Laboratories, West Point, PA Summer 2005 – 2006
Research Summer Intern in Department of Drug Metabolism Supervisor: Thomas Rushmore, Ph.D., Senior Scientist, DMPK
Jackson State University, Jackson, MS October 2004 – May 2006
Undergraduate Research Assistant in Department of Chemistry Mentor: Ken S. Lee, Ph.D., Professor
Vanderbilt University, Nashville, TN May 2004 – August 2004
Undergraduate Research Intern in Department of Pharmacology Mentor: Brian E. Wadzinski, Ph.D., Associate Professor
RICE University, Houston, TX May 2003 – August 2003
Undergraduate Research Intern in Department of Biochemistry and Cell Biology Mentor: Kathleen S. Matthews, Ph.D., Professor and Dean
FUNDING:
Research Support
Title: Defining the molecular mechanisms of lapatinib-induced hepatotoxicity Award: Postdoctoral Research Fellowship
Sponsor: United Negro College Fund (UNCF) – Merck Science Initiative
Amount: $92,000
Date: 9/01/11 – 2/28/13
Role (% Effort): Klarissa D. Hardy, Ph.D. (PI; 100%)
Title: Formation and metabolism of 15-deoxy-Δ12,14-prostaglandin J2in vivo
Award: Ruth L. Kirschstein National Research Service Award (NRSA) Pre-doctoral Fellowship (F31) to Promote Diversity
Sponsor: National Institutes of Health (NIH), National Institute of Aging Date: 3/15/10 – 5/15/11
Role (% Effort): Klarissa D. Hardy (PI; 100%)
HONORS AND AWARDS:
• United Negro College Fund (UNCF) – Merck Science Initiative September 2011 Postdoctoral Science Research Fellowship
• Vanderbilt Institute of Chemical Biology (VICB) Research Symposium August 2010 Award for Oral Presentation
• Pharmacology Department Retreat, Vanderbilt University October 2009 First Place Oral Presentation
• Ruth L. Kirschstein National Research Service Award (NRSA) September 2009 National Institutes of Health (NIH), F31 Individual Pre-doctoral Fellowship
• Society for Free Radical Biology and Medicine (SFRBM) 14th Annual Meeting November 2007 Young Investigator’s Award
• Interdisciplinary Graduate Program, Vanderbilt University - Director’s Award August 2006 • Jackson State University Alumni Association - Jacksonian Award May 2006 • Department of Chemistry, Jackson State University May 2006
Top Senior Chemistry Student Award
• Annual Biomedical Research for Conference for Minority Students (ABRCMS) November 2005 Poster Award in Chemical Sciences
• UNCF–Merck Science Initiative May 2005
Undergraduate Scholarship and Internship
• Department of Chemistry, Jackson State University May 2005 Top Junior Chemistry Student Award
• National Institute of General Medical Sciences (NIGMS) October 2004 Minority Access to Research Careers (MARC) Fellowship Award
• Louis Stoke's Mississippi Alliance for Minority Participation State Conference October 2004 First Place Poster Award
• John A. Peoples Science and Mathematics - Scholarship Award May 2004 • Department of Chemistry, Jackson State University May 2004
TEACHING ACTIVITIES:
• Guest lecturer, Jackson State University – Jackson, MS March 2012 Lecture presentation to undergraduate and graduate chemistry students for the
Department of Chemistry and Biochemistry Seminar Series
• Tutor, Seattle Union Gospel Mission – Seattle, WA 2011-2012 • Guest lecturer, Eicosanoid Core Laboratory, Vanderbilt University – Nashville, TN July 2011
Small group lecture presentation to research associates in the Eicosanoid Core Laboratory to teach the basic principles of eicosanoid biosynthesis and action
• Teaching Fellow, Vanderbilt University – Nashville, TN Fall 2010 Small group discussion leader for the Interdisciplinary Graduate Program for
graduate students to review scientific journal articles and teach experimental approaches in biomedical research
• Tutor, Club Z In-home Tutoring Service – Nashville, TN 2009-2010
Tutor for Vanderbilt University undergraduate student in organic chemistry
and biological science; tutor for Harpeth Hall high school student in general chemistry
• Tutor, Ensworth High School – Nashville, TN 2008-2009
Tutor for high school students (4) in biology, chemistry, and physics
• Tutor, Schrader Lane Church of Christ Tutoring Program – Nashville, TN 2006-2010 Tutor for high students (4) in chemistry and mathematics
LEADERSHIP AND SERVICE: University Committees
• Research Initiative for Scientific Enhancement (RISE) Program, Jackson State University 2012 External Advisory Board
• Pharmacology Graduate Student Association, Vanderbilt University 2010-2011 Vice President of Academic Affairs
Pharmacology Student Forum on Personalized Medicine, Vanderbilt University 2010 Student Organizing Committee
• Vanderbilt Institute of Chemical Biology (VICB) Research Symposium 2010 Student Organizing Committee
• Workshop on Graduate Fellowships, Vanderbilt University 2010 Student Organizer and Presenter
• Workshop on Careers in Biomedical Research, Vanderbilt University 2010 Student Organizer
• Vanderbilt University and Meharry, Pharmacology Departmental Retreat 2009-2010 Student Organizing Committee
• Alliance for Cultural Diversity in Community, Vanderbilt University 2007-2010 Outreach Coordinator
• Martin Luther King, Jr. Commemorative Series, Vanderbilt University 2007-2010 Steering Committee
Science Education Outreach
• Family Science Night at Youth Encouragement Services (YES) April 2010 Organizer and Volunteer
• Boy Scouts’ Science Day with Schrader Lane Boy Scout Troop February 2010 Coordinator and Volunteer
• Family Science Day at Our Savior Lutheran Academy – Volunteer February 2009 • Family Science Night at Dobson Elementary School – Volunteer December 2009 • Family Science Night at Cane Ridge High School – Volunteer November 2008 • Volunteer Scientist-in-the-Classroom Partnership (VSCP) - Organizer 2007-2010 • Vanderbilt University Center for Science Outreach - Volunteer 2007-2010
PROFESSIONAL DEVELOPMENT:
• University of Washington School of Pharmacy, Teaching Certificate in Pharmacy Education June 2012
• Innovations in Research Educational Symposium May 2012 Washington Biotechnology and Biomedical Association
• University of Washington, Teaching and Learning Symposium April 2012
PROFESSIONAL ORGANIZATIONS:
• International Society for the Study of Xenobiotics (ISSX) 2012-present
• Association of UNCF-Merck Fellows (AUMF) 2008-present
• Society for Free Radical Biology and Medicine (SFRBM) 2007-2011
• Phi Kappa Phi Honor Society 2004-2006
SCIENTIFIC PRESENTATIONS AND ABSTRACTS: Poster Presentations
1. Hardy, K.D., Wahlin, M.D., Rettie, A.E., and Nelson S.D. Induction of CYP3A4 by Dexamethasone Enhances the Cytotoxicity of Lapatinib in HepaRG Cells. Abstract for poster presentation. Workshop Organized by the New England Drug Metabolism Discussion Group (NEDMDG) and the International Society for the Study of Xenobiotics (ISSX), Cambridge, MA, April 2013.
2. Hardy, K.D., Wahlin, M.D., Rettie, A.E., and Nelson S.D. Bioactivation by CYP3A plays a critical role in lapatinib-‐induced hepatocellular injury. Abstract for poster presentation. International Society for the Study of Xenobiotics, 18th Annual Regional Meeting, Dallas, TX, October 2012.
3. Wahlin, M.D., Hardy, K.D., et al. Metabolic Activation by CYP3A plays a critical role in lapatinib-‐
induced hepatocellular injury. Poster presentation, Society of Toxicology Annual Meeting, San Francisco, CA, March 2012.
4. Hardy, K.D., Milne, G.L., Yin, H., Roberts, L.J., II. Cyclopentenone Prostglandin 15-Deoxy-Δ12,14 -Prostaglandin J2 is Metabolized in Primary Human Hepatocytes via Conjugation with Glutathione.
Poster presentation, Keystone Symposium – Bioactive Lipids: Biochemistry and Diseases – Kyoto Japan, June 2010.
5. Hardy, K.D., Cox, B.E., Milne, G.L., Yin, H. Morrow, J.D., Roberts, L.J., II. Formation of the Highly Reactive Cyclopentenone Eicosanoid of 15-deoxy-Δ12,14-prostaglandin J2 in Vivo via the Isoprostane
Pathway. Abstract for poster presentation, Society for Free Radical Biology and Medicine (SFRMB) Annual Meeting, San Francisco, CA, November 2009.
6. Hardy, K.D., Milne, G.L., Yin, H. Morrow, J.D., Roberts, L.J., II. Formation of the Highly Reactive Cyclopentenone Eicosanoid of 15-deoxy-Δ12,14-prostaglandin J2 in Vivo via the Isoprostane Pathway.
Abstract for oral presentation. Vanderbilt University - Pharmacology Department Retreat, Cadiz, KY, October 2009.
7. Hardy, K.D., Milne, G.L., Yin, H. Morrow, J.D., Roberts, L.J., II. Formation and Metabolism of 15-deoxy-Δ12,14-prostaglandin J2 in Vivo. Abstract for oral presentation, Annual Graduate Student Research
Symposium, Nashville, TN, March 2009.
8. Hardy, K.D., Milne, G.L., Yin, H., Roberts, L.J.,II, Morrow, J.D. Formation of 15-deoxy-Δ12,14 -prostaglandin J2 (15-d-PGJ2) via Nonenzymatic Oxidation of Arachidonic Acid. Abstract for poster
presentation, Society for Free Radical Biology and Medicine (SFRMB) Annual Meeting, Indiannapolis, IN, November 2008.
9. Hardy, K.D., Dostalek, M., Brooks, J.D., Milne, G.L., Moore, M.M., Sharma, S., Morrow, J.D., Guengerich, F.P. In Vivo Oxidative Damage in Rats is Associated with Barbiturate Response but Not Other Cytochrome P450 Inducers. Abstract for poster presentation, Society for Free Radical Biology and Medicine (SFRMB) Annual Meeting, Washington D.C, November 2007.
10. Hardy, K.D., Eisenhandler, R., Rushmore, T. An LC/MS/MS Method for the Assay of 1’-Hydroxymidazolam and Midazolam in a CYP3A4 Bioreactor. Abstract for poster presentation, Annual Biomedical Research Conference for Minority Students (ABRCMS), Dallas, TX, November 2005.
11. Hardy, K.D., Coffee, L., Adams, D., Wadzinski, B.E. The Role of PP2A in the mTOR/p70 S6 Kinase Signal Transduction Pathway. Abstract for poster presentation, Louise Stoke's Mississippi Alliance for Minority Participation (LSMAMP) – State Conference, Jackson, MS, October 2004.
Invited Podium Presentations
1. Hardy, K.D., Wahlin, M.D., Rettie, A.E., and Nelson S.D. Investigating Mechanisms of Lapatinib-induced Hepatotoxicity in HepaRG Cells. Invited podium presentation. Gordon Research Conference on Drug Metabolism, Holderness, NH, July 2012.
2. Hardy, K.D. Defining the Chemical and Biochemical Mechanisms of Drug-induced Liver Injury. Guest lecturer. Undergraduate and Graduate Chemistry Seminar Series. Jackson State University, Department of Chemistry and Biochemistry, Jackson, MS, March 2012.
3. Hardy, K.D., Milne, G.L., Yin, H., Roberts, L.J., II. Formation and Metabolism of 15-Deoxy-Δ12,14 -Prostaglandin J2. Abstract for podium presentation, Vanderbilt Institute of Chemical Biology (VICB)
Research Symposium, August 2010.
PUBLICATIONS: Research Papers
1. Hardy, K.D., Wahlin, M.D., Papageorgiou, I., Unadkat, J.D., Rettie, A.E., and Nelson, S.D. Studies on the Role of Metabolic Activation in Tyrosine Kinase Inhibitor (TKI)-dependent Hepatotoxicity: Induction of CYP3A4 Enhances the Cytotoxicity of Lapatinib in HepaRG Cells
,
in preparation
.
2. Hardy, K. D., Cox, B. E., Milne, G. L., Yin, H., and Roberts, L. J., II. Nonenzymatic Free Radical-catalyzed Generation of 15-Deoxy-Δ12,14-Prostaglandin J2-like Compounds (Deoxy-J2-Isoprostanes) in Vivo. (2011) Journal of Lipid Research, 52(1):113-124.
3. Dostalek, M., Hardy, K. D., Milne, G. L., Morrow, J. D., Chen, C., Gonzalez, F. J., Gu, J., Ding, X., Johnson, D. A., Johnson, J. A., Martin, M. V., and Guengerich, F. P. Development of oxidative stress by cytochrome P450 induction in rodents is selective for barbiturates and related to loss of pyridine nucleotide-dependent protective systems. (2008) Journal of Biological Chemistry, 283(52):17147-57. 4. Dostalek, M., Brooks, J. D., Hardy, K. D., Milne, G. L., Moore, M. M., Sharma, S., Morrow, J. D., and
Guengerich, F. P. In vivo oxidative damage in rats is associated with barbiturate response but not other cytochrome P450 inducers. (2007) Molecular Pharmacology, 72(6):1419-24.
Book Chapters and Reviews
1. Hardy, K. D., Milne, G. L., and Roberts, L. J., II. The Research and Biology of Cancer. “Eicosanoids: Lipid Mediators and Biomarkers in Cancer.” iConcept Press, in preparation.
2. Milne, G. L., Yin, H., Hardy, K. D., Davies, S. S., and Roberts, L. J., II. Isoprostane Generation and Function. (2011) Chemical Reviews, 111(10):5973-5996.
3. Brooks, J. D., Cox, B. E., Hardy, K. D., Sanchez, S. C., Koestner, T. R., Howard, J. R., and Milne, G. L. Biomarkers for Antioxidant Defense and Oxidative Damage – Principles and Practical Applications. “The Isoprostanes: Accurate Markers and Potent Mediators of Oxidant Injury in Vivo”. October 2010. Wiley-Blackwell, 65-84.
