Vigour Committee. Alison A Powell University of Aberdeen Aberdeen, UK. Update of Vigour Committee activity 2014

Vigour Committee

Alison A Powell

University of Aberdeen Aberdeen, UK

• Update on membership

• Test development:

Introduction of new methods

Introduction of new species

• Radicle emergence test • Conductivity test

Shortening of Controlled Deterioration test

• Other vigour related activities

Vigour Committee members

Ibrahim Demir Turkey

Bob Elliot Canada

Dionysia Fasoula Cyprus Carina Gallo Argentina

Simon Goertz Germany

Mohammad Khajeh-Hosseini Iran

Tim Loeffler United States Stan Matthews United Kingdom Gillian McLaren United Kingdom Marie-Helene Wagner France

Yan Wang China

Enrico Noli Italy Carey Mathiessen Canada Manjunath Prasad India

Test development: Introduction of new method

Tetrazolium staining as a vigour test for Glycine max

• WG leader : Carina Gallo (AR)

• Comparative test results now complete

• Participants:

Miriam Arango, Argentina

Maria Victoria Francomano, Argentina Claudia Carracedos, Argentina

Jose de Barros Franca-Neto, Brazil Odair Costa, Brazil

Tana Moreano, Brazil

Test development: Introduction of new species

Stages of test development

• Assess RE at range of times in the laboratory

• Complete field emergence trials if evidence of RE vs FE relationship not available

• Select time of RE assessment that best predicts FE

• Carry out comparative test.

RE test:

RE assessed at range of times

using

automated germination curves

• Marie-Hélène Wagner: GEVES

• Automated germination time courses determined for:

Sunflower at 25oC

Cabbage at 20o_C Onion at 20o_C Wheat at 20o_C Lucerne at 20o_C

0 20 40 60 80 100 0 6 12 18 24 30 36 42 48 54 60 66 72 Ge rm in ation (% )

Imbibition time (hours)

Sunflower at 25°C Mean Min Max 0 20 40 60 80 100 0 12 24 36 48 60 72 84 96 108 120 132 Ge rm in ation (% )

Imbibition time (hours)

Onion at 20°C Mean Min Max 0 20 40 60 80 100 0 12 24 36 48 60 72 Ger m in ati on (% )

Imbibition time (hours)

Cabbage at 20°C Mean Min Max 0 20 40 60 80 100 0 12 24 36 48 60 72 84 96 108 120 Ge rm in ation (% )

Imbibition time (hours)

Wheat at 20°C

Mean Min Max

RE test: Onion

• WG leader: Hulya Ilbi (TR), assisted by Gülgün Baslar

• 16 Seed lots evaluated for:

Radicle emergence (single counts of RE made 10 times from 24 hours to 185 hours)

Normal germination

• Field emergence in progress

• Comparative test to take place after relationship with FE established

RE test: Leek

• WG leader: Ibrahim Demir (TR)

• 21 seed lots evaluated for:

Radicle emergence (single counts of RE made 11 times from 64 hours to 160 hours)

Normal germination

• Field emergence complete; FE related to RE

RE test: Radish

WG leaders: Stan Matthews (UK), Kazim Mavi, (TR)

• RE timing and relation to FE previously established

• 6 seed lots (>90% NG) selected from 10 tested for comparative test

Participants

• Hulya Ilbi (TR)

• Ibrahim Demir (TR)

• Gillian McLaren (UK)

• Marie-Hélène Wagner (FR)

RE test: wheat and alfalfa

• WG leader: Mohammad Khajeh Hosseini

• Contacted :

100 wheat seed companies in Iran contacted

Alfalfa seed companies and public research centres

• Seed lots for the initial tests received

• Two PhD students trained in seed quality assessment, particularly vigour methods.

RE test:

Elymus nutans, Avena sativa

• WG leader: Yanrong Wang (CN)

• 2012: Germination and field tests for E. Nutans

Field emergence correlated with MGT and RE tests

• 2014: Germination progress curves determined:15 lots of both species

• 2014 : 2 field sowings to be completed for each species; relationship to RE examined

Test development: Introduction of new species

Conductivity test

Chickpea: Cicer arietinum – Desi type Radish : Raphanus sativus

all lots R²= 0.877*** excluding R7 R 2_{= 0.856**} y = -0.2235x + 123.32 40 50 60 70 80 90 100 90 140 190 240 290 Se e d lin g e m e rg e n c e (% ) EC after 17 hours (µS cm-1 _g-1₎ A all lots R² = 0.865*** excluding R7 R 2_{= 0.789**} y = -0.3167x + 131.05 0 20 40 60 80 100 90 140 190 240 290 S ta n d a rd g e rm in a tio n a ft e r st o ra g e ( % ) EC after 17 hours (µS cm-1 _g-1₎ B

EC test and Brassicaceae: Raphanus sativus (radish) Bulk EC test- 4 x 100 seeds

Emergence

12 months storage (25o_C)

Mavi, Mavi, Demir and Matthews, SST, 2014

EC predicts vigour:

• emergence

EC: Radish

• Clear differences in EC of same lots as used or RE test

• Both EC and RE being tested in same round of comparative testing

Participants

• Hulya Ilbi (TR)

• Ibrahim Demir (TR)

• Gillian McLaren (UK)

• Marie-Hélène Wagner (FR)

Shortening the CD test

• WG leader Marie-Hélène Wagner (FR)

• Two approaches

Alternative method of raising and equilibrating seed MC

Replacing germination test after deterioration with an EC test

• Comparative test completed: 10 labs , 5 lots oilseed rape Participants:

Frédéric Corre (FR) Malavika Dadlani (IN) Ibrahim Demir (TR) Hulya Ilbi (TR)

Mohammad Khajeh Hosseini, (IR) Gillian McLaren (UK) Carey Matthiessen (CA) Enrico Noli (IT)

Yan Rong Wang (CN) Marie-Hélène Wagner (FR)

Comparative test

• Raising seed MC

5 labs : MC raised using filter paper method

4 labs: MC raised using alternative method (roller method)

1 lab: added water to foil packet

• After CD all labs completed both

EC test (4 x 100 seeds in 40 ml)

Other development work

• Previously: SBP related to SG, EC and CD in artificially aged seeds

• Promising relationships less interesting in lots varying in quality after natural

storage

1. Seed biotinylated proteins (M-H Wagner [FR])

Other Vigour Committee activities

Proficiency test:

• Organiser: Gillian McLaren (UK)

• 73 laboratories

Workshop:

In memoriam

Jan. 1, 1932-Sept 12, 2013

• Accelerated ageing vigour test with J Curt Delouche in 1960’s

• Agronomist with Mississippi Agricultural Experiment Station, Professor of Agronomy with Mississippi State University,

Seed Technology consultant

• Known globally for his contribution to cottonseed production and quality evaluation. –

Thank you!

• All members of Vigour Committee

• Nadine Ettel, TCOM co-ordinator

• ECOM for financial support for:

RE test development for:

• Wheat, alfalfa, onion, radish, Elymus nutans and Avena sativa

EC test development for: