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Designing a laser scanning confocal microscope: a new scan engine layout

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Figure 1.1: Comparison of conventional wide field microscope [left] to confocal microscopy [right].Source: http://www.microscopyu.com/ [1].
Figure 1.2: Principle light pathways in confocal microscopy, transmission mode. This is not a full LSCM,only a confocal microscope’s principles.
Figure 1.3: The configuration of the scanning system of a LSCM. G: Galvanometer; S: scan-lens; BFP:back focal plane; T: tube-lens; f1: focal length of S; f2: focal length of T [8].
Figure 2.1: Lay out of the scan engine and its mirrors: X1, Y1, X2 and Y2. In this setting the scan areawould be in the z, x plane but this can easily be changed by adding another 45◦mirror to convert it to thex, y plane
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