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In Vitro and In Vivo Evaluation of 89Zr-DS-8273a as a Theranostic for Anti-Death Receptor 5 Therapy

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Academic year: 2020

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Figure 1: In vitro binding assays with radiolabeled DS-8273a. Lindmo plots showing binding of (A) 111In-CHX-A″-DTPA-DS-8273a and (B) 89Zr-Df-Bz-NCS-DS-8273a to increasing concentrations of DR5-positive COLO 205 cells
Figure 4: In vivoPET/MRI  imaging of DR5 in COLO 205 xenografted mice. (A)Representative whole-body (left) and transaxial (right) images of CT (top row), SPECT (middle row) and fused SPECT/CT (bottom row) taken on day 7 post injections of 111In-CHX-A″-DTPA
Figure 5: Influence of cold dose DS-8273a on tumor uptake of Psizes of each dose level (E) on day 2, but significant differences in tumor size were observed on day 7 post injection
Table 1: Pharmacokinetic parameters of 89Zr- and 111In-labeled DS-8273a.

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