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Mechanical properties of paraformaldehyde-treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy

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Academic year: 2020

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Figure

Fig. 1 Schematic view of principle of SPM techiques and L929 cell surface images using SICM
Fig. 2 a Schematic view of the cell surface fluctuation setting by SICM. To investigate fluctuation levels, the nano‑pipette was positioned on the apex of the single cell
Fig. 4 a Optical image of AFM tips positioning on the target cell. b Typical force‑separation curve for a fixed cell (4% PFA‑treated cell), live cell, and solid substrate
Fig. 6 Evaluation of PFA‑mediated cytotoxicity on L929 cells. dead cell ratio dependent on the titration of a Fluorescence microscope images for assessment of live and dead cell ratio depend‑ent on titration of CPFA

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