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Embryonic stem cells stably transfected with mRAR beta 2 lacZ exhibit specific expression in chimeric embryos

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Figure

Fig. 1. Structureof the mRARI\-lacZtransientexpressioncassette(AI and itsexpressionin P19 ECcelis(B)
Fig. 2. Southernhybridizationof stablytransfectedES celllines.Ten Jig of genomicDNAfromthreeindependentclones(2£5,2F3 and 1F5) as wellas negative control DNA from non-transfected ES cells (£14) were loaded per lane
Fig. 3. mRARB -lacZ expressionin stableES clonesis specificallyinduced by RA. Three stable clones (2E5, 2F3, and 1F5) were maintainedin BRL-CM
Fig. 4. mRARB .lacZ expressionin chimericmouseembryos.ES cells resulting from stable transfectionof the mRARf3 -lacZ constructwereinrroducedintoembryos b~blastocyst injection
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