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In situ hybridization with non radioactive digoxigenin 11 UTP labeled cRNA probes: localization of developmentally regulated mouse tenascin mRNAs

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Figure

Fig. 1. Structuralmodel of mouse tenascinand alignmentsof RNAprobes. TN has a signal peptide(5), a sectorof central globule (G) and ana.heJical structure(H)at the N-terminus.141/2 EGF-likerepeats(EGFJ and13 FN type/If-likerepeats(FNII/Jfollow
Fig. 2. Northernblotillustratingtissue-specificexpressionof TNmRNA isoforms.10/19 of total RNAs from P2 mice were electrophoresedin formaldehyde agarose gels, transferred to a nitrocellulosemembrane,and hybridized
Fig. 3. Signal intensity depends on proteinaseKdigestion. Sections of P2 cerebellumlA-F)and lung (G.L) were digestedwith 8 IA
Fig. 4. Signalintensitydependson probelengthand TN cDNA regionrepresented.Six differentprobes(5', EGF, FN1-5, FNAE, FN11.13,and Fbg)were testedindividually in hybridization reactions,and a mixture of these probes.MTN-, was tested separately.No signal was o

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