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Developmental incompatibility between cell nucleus and cytoplasm as revealed by nuclear transplantation experiments in teleost of different families and orders

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Figure

Fig. 2. Photographshowsspeciesand sexualbryanus.thedifferentfishusedin bothnucleartransplantation hybridizationexperiments.fa) Gold-fish 1Carassiusauratus,familyCyprinidae.orderCypriniformes).(b) Loach (Paramisgurnusda-familyCobiridae.order CypriniformesJ.(e) Tllapia !Oreochromisnilotlca.family Cichlidae,orderPerciformes).
Fig.4.tweenNucleartransplantationexperimentsbe-differentordersof teleosts.Earlydevelop-mentof NCH embryosobtainedfromthe combinationof nucelusof filapia ~Oreochromlsnilotica.order Pecl-formes)and the cytoplasmof goldfish(Carasslusaura-tus, order, cypriniformesJ.(a) Isolatedblastulacells ofTllapia whichwere used as nucleusdonors(arrow).(bl2-cell, Ie) 4-cell, (d) 8-cell, (e) 32-cell,lfIlateblastula,(gla metaphaseofhybndblastula.Twolongchromo-somes are markerchromosomesof Tllapla
Fig.5. ThechromlSscanningelectronmicroscopicpicturesofnormalgoldfish(Carass;usauratus)blastulaandNCHblastulaobtainedfrom the combinationof Tilapia (Oreo-nilotlca~ nucleusand goldfishcytoplasm,(al Nor-mal goldfishblastula(x200)

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