From the spectrin gene to the assembly of the membrane skeleton

When mixtures of af, and afi' dimers are bound to spectrin- depleted inside-out membrane vesicles from normal cells and tetramers are allowed to form by equilibration at 30°C, the

ratio), erythrocyte spectrin content (measured by radioimmu- noassay), the membrane shear modulus, the creasing pressure and the whole cell recovery time constant were determined for..

of: (a) the relationship between our in vitro assay and the in vivo situation; (b) storage-related changes in red cell phospholipid loss and membrane function; and (c) similarities

that actin filaments in unstimulated, discoid platelets require high g forces (100,000 g for 3 h) to be sedimented from Triton X-100 lysates. In contrast, in platelets that have lost

We have examined the association of spectrin, the major skeletal protein, with ankyrin, its high-affinity membrane binding site, and found sickle erythrocytes to have markedly reduced

(asymptomatic mothers of the two patients) exhibited a mild but reproducible increase of spectrin dimers in 0 degrees C extracts and a defective reassociation of spectrin dimers of

To test this we first incubated erythrocytes for 1 h at 37°C in Tris-maleate buffers at pH 5, 6, 7, anl 8, and observed the effect of the pH change on erythrocyte shape, intracellular

To test whether ␣ -Spectrin is similarly required for the stability of ␤ -Spectrin protein, we generated homozygous-mutant eye discs lacking ␣ -spectrin expression.. In these

The slope for gene 49 is half of that for gene 46; compared with the decrease in burst size, the decrease in the recombinant frequency was less sensitive to limiting

Effect of Contact Time: The uptake of fluoride from industrial waste water by multani matti and red soil the adsorption rapidly increased in the first 20 minutes after 20

members of the microbiome have key roles in health and resilience (e.g. The inoculation of corals with probiotics isolated from coral tissue and sur- rounding water has been shown to

The simulation of the deformation of the RBC aspirated into the micropipette was first performed for three initial geometries (two concentric circles, a biconcave shape and a sphere)