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Integration and Characterization of T-DNA Insertion in Upland Cotton

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Figure 1. Map of the T-DNA region with binary vector pCAMBIA2300 for cotton callus transformation (see GenBank accession AF234315)RB and LB represent the left border and the right border, respectively; L1-3 and R1-3 represent the Tail-PCR pri-mers adjacent to LB and RB; numbers 5/6 are used to identify the positive transformants and used as hybridization probe, and 7/8 was used as real-time quantitative PCR primers for the analysis of transgenic copies; the arrows indicate the direction of primers from the 5' to the 3' end
Figure 2. Identification of positive transgenic cotton plants: (a) kanamycin-sensitive, (b) kanamycin-resi-stant plants, (c) shows a conventional PCR of nptII M: marker; 1: positive plasmid control, 2: non-transgenic plant, 3–8: transgenic plants
Table 1. Copy numbers of the nptII gene in 92 transge-nic plants
Table 2. Variation of T-DNA border adjacent regions in transgenic cotton plants
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