SpringerBriefs in Applied Sciences and Technology

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SpringerBriefs in Applied Sciences and Technology

Series editors

Amit Kumar, Hyderabad, India Allam Appa Rao, Hyderabad, India

Forensic and Medical Bioinformatics

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More information about this series at http://www.springer.com/series/11910

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Samira Hosseini

^•

Patricia V ázquez-Villegas

Marco Rito-Palomares

^•

Sergio O. Martinez-Chapa

Enzyme-linked

Immunosorbent Assay (ELISA)

From A to Z

123

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Samira Hosseini

Escuela de Ingeniería y Ciencias Tecnologico de Monterrey Monterrey, NL

Mexico

Patricia Vázquez-Villegas Escuela de Ingeniería y Ciencias Tecnologico de Monterrey Monterrey, NL

Mexico

Marco Rito-Palomares

Escuela de Medicina y Ciencias de Salud Tecnologico de Monterrey

Monterrey, NL Mexico

Sergio O. Martinez-Chapa Escuela de Ingeniería y Ciencias Tecnologico de Monterrey Monterrey, NL

Mexico

ISSN 2191-530X ISSN 2191-5318 (electronic)

SpringerBriefs in Applied Sciences and Technology

ISSN 2196-8845 ISSN 2196-8853 (electronic)

SpringerBriefs in Forensic and Medical Bioinformatics

ISBN 978-981-10-6765-5 ISBN 978-981-10-6766-2 (eBook)

https://doi.org/10.1007/978-981-10-6766-2 Library of Congress Control Number: 2017962065

This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, speciﬁcally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microﬁlms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed.

The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a speciﬁc statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.

The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional afﬁliations.

Printed on acid-free paper

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The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore

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Preface

ELISA: From A to Z was written aiming to provide Readers with a proper coverage of all aspects of ELISA from fundamentals of immune system and the history of the analytical assay prior to the invention of ELISA to the materials of choice for fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters among the rest. This book guides Readers through different steps of the analytical assay while familiarizing them with the possible sources of error in the assay. The book offers detailed insights on the immobi- lization techniques used for protein attachment, different methods for evaluation of the assay and calculation of the key important parameters in the analytical assay such as sensitivity, speciﬁcity, accuracy, and limit of detection. Advantages and shortages of the conventional ELISA as well as different attempts for improvement of its performance are covered in this book. Merging and intergrading different technologies with widely known ELISA have opened numerous windows of opportunity to the advancement of this immunoassay. In that respect, the current book provides the latest updates on integrated platforms such as ELISpot, plas- monic ELISA, sphere-/bead-based ELISAs, paper-/ﬁber-based ELISAs as well as ELISA in micro-devices.

Monterrey, Mexico Samira Hosseini

Patricia Vázquez-Villegas Marco Rito-Palomares Sergio O. Martinez-Chapa

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Acknowledgements

Authors would like to acknowledge the ﬁnancial support of Tecnológico de Monterrey, Mexico, for the special grant (grant number: 002EICII01) awarded by the Sensors and Devices Focus Group, School of Engineering and Sciences, Tecnológico de Monterrey, Monterrey, Mexico.

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SpringerBriefs in Applied Sciences and Technology