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Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication

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Figure

Fig. 1. The CMG DNA helicase is ubiquitylated on its Mcm7 subunit) Extracts of S-phase cells (YMM22 and YMM23), with or without a plasmid expressing protease before denaturation with 8 M urea (described in the materials and methods section), so that ubiqui
Fig. 2. SCFDia2( drives in vitro ubiquitylation of CMG in yeast cell extractsA) Control (YHM117) and TAP-SLD5 cells (YHM132) were processed as above, except
Fig. 4. CMG is present during the G1components of the replisome progression complex such as Csm3 and Ctf4 (1), was monitored by immunoblotting of the indicated proteins
Fig. 6. Dia2 induces disassembly of terminated CMG complexes but does not prevent assembly of active CMG complexes(weak and inducible galactose (pheromone (stage 1)

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