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Senescence of human vocal fold fibroblasts in primary culture

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Figure

Figure 1. An example of PCR agarose gel, showing PCR products gener-ated from RNA extracted from primary-culture human vocal fold fibro-blasts of passages 1-5 (P1-P5) and passage 10 (P10) for the hyaluronidase I target primer as shown in Table 1 (MW=100 ba
Figure 6. Passage 5 (P5) karyotype: (A) Triploidy of chromosome 3 as seen in P1-P4 (boxed area); (B) An additional clone was detected displaying +8 abnor-mality (circled area)
Figure 8. Cumulative population doubling time (PDT) of pri-mary vocal fold fibroblasts as a function of passaging (means ± standard error of the mean; n=3)
Figure 9. Densitometric ratios for select extracellular matrix target genes (means ± standard deviations; n=3), showing the in-the standard control (GAPDH): (A) MMP-1tegrated density volumes (IDV) or cumulative grey scale values of PCR agarose gel bands fo

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